Genetic diversity creation is a core technology in directed evolution where a high quality
mutant library is crucial to its success. Owing to its importance, the technology in genetic …

The wealth of distinct enzymatic functions found in nature is impressive and the on‐going
evolutionary divergence of enzymatic functions continues to generate new and efficient …

The ability to engineer natural proteins is pivotal to a future, pragmatic biology. CRISPR
proteins have revolutionized genome modification, yet the CRISPR-Cas9 scaffold is not …

Abstract Domain recombination is a key principle in protein evolution and protein
engineering, but inserting a donor domain into every position of a target protein is not easily …

Efforts to engineer microbial factories have benefitted from mining biological diversity and
high throughput synthesis of novel enzymatic pathways, yet screening and optimizing …

Monitoring the aggregation of proteins within the cellular environment is key to investigating
the molecular mechanisms underlying the formation of off-pathway protein assemblies …

Naturally evolved metabolite-responsive biosensors enable applications in metabolic
engineering, ranging from screening large genetic libraries to dynamically regulating …

Deep mutational scanning has been used to create high-resolution DNA sequence maps
that illustrate the functional consequences of large numbers of point mutations. However …

Adenylate kinase (AK) orthologs with a range of thermostabilities were subjected to random
circular permutation, and deep mutational scanning was used to evaluate where new …

Bacteriophytochrome photoreceptors (BphP) are knotted proteins that have been developed
as near-infrared fluorescent protein (iRFP) reporters of gene expression. To explore how …