Challenges in the use of sortase and other peptide ligases for site-specific protein modification
HE Morgan, WB Turnbull, ME Webb - Chemical Society Reviews, 2022 - pubs.rsc.org
Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …
many challenges associated with the development of protein modification techniques, in …
Chiral posttranslational modification to lysine ε-amino groups
C Moreno-Yruela, M Bæk, F Monda… - Accounts of chemical …, 2022 - ACS Publications
Conspectus The sophistication of proteomic analysis has revealed that protein lysine
residues are posttranslationally modified by a variety of acyl groups. Protein lysine …
residues are posttranslationally modified by a variety of acyl groups. Protein lysine …
Acetylation of histone H2B marks active enhancers and predicts CBP/p300 target genes
Chromatin features are widely used for genome-scale mapping of enhancers. However,
discriminating active enhancers from other cis-regulatory elements, predicting enhancer …
discriminating active enhancers from other cis-regulatory elements, predicting enhancer …
Chemical synthesis of post-translationally modified H2AX reveals redundancy in interplay between histone phosphorylation, ubiquitination, and methylation on the …
The chemical synthesis of homogeneously modified histones is a powerful approach to
quantitatively decipher how post-translational modifications (PTMs) modulate epigenetic …
quantitatively decipher how post-translational modifications (PTMs) modulate epigenetic …
Structural basis of sirtuin 6-catalyzed nucleosome deacetylation
The reversible acetylation of histone lysine residues is controlled by the action of
acetyltransferases and deacetylases (HDACs), which regulate chromatin structure and gene …
acetyltransferases and deacetylases (HDACs), which regulate chromatin structure and gene …
Uncoupling histone modification crosstalk by engineering lysine demethylase LSD1
Biochemical crosstalk between two or more histone modifications is often observed in
epigenetic enzyme regulation, but its functional significance in cells has been difficult to …
epigenetic enzyme regulation, but its functional significance in cells has been difficult to …
KATs off: Biomedical insights from lysine acetyltransferase inhibitors
Lysine acetyltransferase (KAT) enzymes including the p300, MYST, and GCN5 families play
major roles in modulating the structure of chromatin and regulating transcription. Because of …
major roles in modulating the structure of chromatin and regulating transcription. Because of …
Thioester‐Assisted Sortase‐A‐Mediated Ligation
C Zuo, R Ding, X Wu, Y Wang, GC Chu… - Angewandte …, 2022 - Wiley Online Library
Sortase A (SrtA)‐mediated ligation, a popular method for protein labeling and semi‐
synthesis, is limited by its reversibility and dependence on the LPxTG motif, where “x” is any …
synthesis, is limited by its reversibility and dependence on the LPxTG motif, where “x” is any …
Genetic Code Expansion Approaches to Decipher the Ubiquitin Code
The covalent attachment of Ub (ubiquitin) to target proteins (ubiquitylation) represents one of
the most versatile PTMs (post-translational modifications) in eukaryotic cells. Substrate …
the most versatile PTMs (post-translational modifications) in eukaryotic cells. Substrate …
Promotion of RNF168‐Mediated Nucleosomal H2A Ubiquitylation by Structurally Defined K63‐Polyubiquitylated Linker Histone H1
The chemical synthesis of histones with homogeneous modifications is a powerful approach
for quantitatively deciphering the functional crosstalk between different post‐translational …
for quantitatively deciphering the functional crosstalk between different post‐translational …