Cell culture is an important tool for biological research. Two-dimensional cell culture has
been used for some time now, but growing cells in flat layers on plastic surfaces does not …

Moving from cell monolayers to three-dimensional (3D) cultures is motivated by the need to
work with cellular models that mimic the functions of living tissues. Essential cellular …

Cell culture in two dimensions has been routinely and diligently undertaken in thousands of
laboratories worldwide for the past four decades. However, the culture of cells in two …

Extracellular matrix is a key regulator of normal homeostasis and tissue phenotype 1.
Important signals are lost when cells are cultured ex vivo on two-dimensional plastic …

The in vitro analysis of intestinal epithelium has been hampered by a lack of suitable culture
systems. Here we describe robust long-term methodology for small and large intestinal …

Cancer cells exist in a constantly evolving tissue microenvironment of diverse cell types
within a proteinaceous extracellular matrix. As tumors evolve, the physical forces within this …

Life science research focuses on deciphering the biochemical mechanisms that regulate cell
proliferation and function and largely depends on the use of tissue culture methods in which …

In the current pandemic, scenario the world is facing a huge shortage of effective drugs and
other prophylactic medicine to treat patients which created havoc in several countries with …

Experimental models indicate that tumor cells in suspension, unlike solid tumor fragments,
might be unable to produce life-threatening cancer outgrowth when transferred to animal …

Organoids are 3D miniature versions of organs produced from stem cells derived from either
patient or healthy individuals in vitro that recapitulate the actual organ. Organoid technology …