The ability to design efficient enzymes from scratch would have a profound effect on
chemistry, biotechnology and medicine. Rapid progress in protein engineering over the past …

Biocatalysis has become an important aspect of modern organic synthesis, both in
academia and across the chemical and pharmaceutical industries. Its success has been …

Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

The need for understanding essential recognition events in chemistry and biology has
directed considerable efforts toward the development of chemical probes. Fluorescent …

Cellulose is the most abundant renewable natural biological resource, and the production of
biobased products and bioenergy from less costly renewable lignocellulosic materials is …

Successful evolutionary enzyme engineering requires a high throughput screening or
selection method, which considerably increases the chance of obtaining desired properties …

Metabolic engineering for the overproduction of high-value small molecules is dependent
upon techniques in directed evolution to improve production titers. The majority of small …

Directed evolution and rational design are powerful strategies in protein engineering to tailor
enzyme properties to meet the demands in academia and industry. Traditional approaches …

Genetic sensors capable of converting key metabolite levels to fluorescence signals enable
the monitoring of intracellular compound concentrations in living cells, and emerge as an …