▪ Abstract DNA topoisomerases solve the topological problems associated with DNA
replication, transcription, recombination, and chromatin remodeling by introducing …

Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo,
DNase or RNase, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes. In …

We discuss basic physical–chemical principles underlying the formation of stable
macromolecular complexes, which in many cases are likely to be the biological units …

Integration, excision, and inversion of defined DNA segments commonly occur through site-
specific recombination, a process of DNA breakage and reunion that requires no DNA …

The helix-turn-helix (HTH) domain is a common denominator in basal and specific
transcription factors from the three super-kingdoms of life. At its core, the domain comprises …

On the basis of a structural analysis of 240 protein-DNA complexes contained in the Protein
Data Bank (PDB), we have classified the DNA-binding proteins involved into eight different …

Posttranslational modification by phosphorylation is a ubiquitous regulatory mechanism in
both eukaryotes and prokaryotes. Intracellular phosphorylation by protein kinases, triggered …

The use of Cre/loxP recombination in mammalian cells has expanded rapidly. We describe
here that Cre expression in cultured mammalian cells may result in a markedly reduced …

The three-dimensional structure of a 70-kilodalton amino terminally truncated form of human
topoisomerase I in complex with a 22–base pair duplex oligonucleotide, determined to a …

The genome of the broad host range Streptomyces temperate phage, φC31, is known to
integrate into the host chromosome via an enzyme that is a member of the …