After decades of research, our knowledge of the complexity of cancer mechanisms,
elegantly summarized as 'hallmarks of cancer', is expanding, as are the therapeutic …

Synthetic biology has mainly focused on introducing new or altered functionality in single
cell systems: primarily bacteria, yeast, or mammalian cells. Here, we describe the extension …

The stable incorporation of transgenes and recombinant DNA material into the host genome
is a bottleneck in many bioengineering applications. Due to the low efficiency, identifying the …

Animals integrate sensory information from the environment and display various behaviors
in response to external stimuli. In Caenorhabditis elegans hermaphrodites, 33 types of …

I outline a streamlined method to insert large, single-copy transgenes into the
Caenorhabditis elegans genome using recombination-mediated cassette exchange (RMCE) …

The identification and characterization of genomic safe harbor sites (GSHs) can facilitate
consistent transgene activity with minimal disruption to the host cell genome. We combined …

High-throughput transgenesis using synthetic DNA libraries is a powerful method for
systematically exploring genetic function. Diverse synthesized libraries have been used for …

Abstract 53BP1 plays a crucial role in regulating DNA damage repair pathway choice and
checkpoint signaling in somatic cells; however, its role in meiosis has remained enigmatic …

A core tenet of synthetic biology is that well-characterized regulatory elements are essential
for engineering biological systems. Here, we characterize the specificity and expression …

Caenorhabditis elegans gut and cuticle produce a disruptive amount of autofluorescence
during imaging. Although C. elegans autofluorescence has been characterized, it has not …