[FeFe]-hydrogenases are complex metalloenzymes, key to microbial energy metabolism in
numerous organisms. During anaerobic metabolism, they dissipate excess reducing …

Pathogenic microorganisms use various mechanisms to conserve energy in host tissues
and environmental reservoirs. One widespread but often overlooked means of energy …

Growing concerns over greenhouse gas emissions and energy insecurity caused by the
depletion of conventional fuels have led to a search for sustainable fuel alternatives. As an …

The newly isolated Clostridium beijerinckii [FeFe]-hydrogenase CbA5H was characterized
by Fourier transform infrared spectroscopy coupled to enzymatic activity assays. This …

We present in this work a kinetic model of the acetone-butanol-ethanol (ABE) fermentation
based on enzyme kinetics expressions. The model includes the effect of the co-substrate …

Background Bio-hydrogen production via dark fermentation of low-value waste is a potent
and simple mean of recovering energy, maximising the harvesting of reducing equivalents to …

[FeFe]-hydrogenase (HydA) is an active biocatalytic enzyme for solar-to-hydrogen (H2)
conversion. However, stability is a main challenge that limits practical applications. This …

Abstract The [FeFe]-hydrogenase CpHydA from Clostridium perfringens was immobilized by
adsorption on anatase TiO 2 electrodes for clean hydrogen production. The immobilized …

A conserved cysteine located in the signature motif of the catalytic center (H-cluster) of
[FeFe]-hydrogenases functions in proton transfer. This residue corresponds to C298 in …

The use of enzymes to generate hydrogen, instead of using rare metal catalysts, is an
exciting area of study in modern biochemistry and biotechnology, as well as biocatalysis …