[HTML][HTML] A comparative analysis of single-cell transcriptomic technologies in plants and animals

VR Netla, H Shinde, G Kumar, A Dudhate, JC Hong… - Current Plant …, 2023 - Elsevier
The development of sequencing methods has resulted in the investigation of many
unexplored research areas. Among the different sequencing methods, single-cell …

Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor

V Gligorovski, A Sadeghi, SJ Rahi - Nature Communications, 2023 - nature.com
The ability to independently control the expression of different genes is important for
quantitative biology. Using budding yeast, we characterize GAL1pr, GALL, MET3pr, CUP1pr …

Convergence of flow cytometry and bacteriology. Current and future applications: a focus on food and clinical microbiology

R Marcos-Fernández, B Sánchez, L Ruiz… - Critical reviews in …, 2023 - Taylor & Francis
Since its development in the 1960s, flow cytometry (FCM) was quickly revealed a powerful
tool to analyse cell populations in medical studies, yet, for many years, was almost …

Genome-wide gene expression noise in Escherichia coli is condition-dependent and determined by propagation of noise through the regulatory network

A Urchueguía, L Galbusera, D Chauvin… - PLoS …, 2021 - journals.plos.org
Although it is well appreciated that gene expression is inherently noisy and that
transcriptional noise is encoded in a promoter's sequence, little is known about the extent to …

Alteration of DNA supercoiling serves as a trigger of short-term cold shock repressed genes of E. coli

S Dash, CSD Palma, ISC Baptista… - Nucleic Acids …, 2022 - academic.oup.com
Cold shock adaptability is a key survival skill of gut bacteria of warm-blooded animals.
Escherichia coli cold shock responses are controlled by a complex multi-gene, timely …

Dielectrophoretic Capture and Electrochemical Enzyme‐Linked Immunosorbent Assay of Single Melanoma Cells at an Array of Interlocked Spiral Bipolar Electrodes

MJ Clark, HJ Moser, RK Anand - ChemElectroChem, 2024 - Wiley Online Library
Abstract Analysis of single cancer cells is critical to obtain accurate patient diagnosis and
prognosis. In this work, we report the selective dielectrophoretic capture and electrochemical …

Stress-Induced Constraint on Expression Noise of Essential Genes in E. coli

PA LaBoone, R Assis - Journal of Molecular Evolution, 2024 - Springer
Gene expression is an inherently noisy process that is constrained by natural selection. Yet
the condition dependence of constraint on expression noise remains unclear. Here, we …

Rapid high-resolution detection of colistin resistance in Gram-negative bacteria using flow cytometry: a comparison with broth microdilution, a commercial screening …

O Ekelund, MA Klokkhammer Hetland… - Journal of …, 2021 - academic.oup.com
Background Even though both EUCAST and CLSI consider broth microdilution (BMD) as the
reference method for antimicrobial susceptibility testing (AST) of colistin, the method exhibits …

Quantum measurement enables single biomarker sensitivity in flow cytometry

J Sabines-Chesterking, IA Burenkov, SV Polyakov - Scientific Reports, 2024 - nature.com
We present the first unambiguous experimental method enabling single-fluorophore
sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a …

[HTML][HTML] A strategy for quantifying microplastic particles in membrane filtration processes using flow cytometry

RJ LaRue, S Koo, A Warren, YG McKay, DR Latulippe - Chemosphere, 2024 - Elsevier
Microplastic (MP) pollution is ubiquitous in the aquatic environment, with significant
quantities of MPs originating from municipal wastewater treatment plants. Efforts to evaluate …