Recent advances in design of fluorescence-based assays for high-throughput screening

X Fang, Y Zheng, Y Duan, Y Liu, W Zhong - Analytical chemistry, 2018 - ACS Publications
Significant improvements in disease management have been achieved in the past few
decades. Using cancer as an example, survival rates have increased dramatically in …

Emergent biosensing technologies based on fluorescence spectroscopy and surface plasmon resonance

A Camarca, A Varriale, A Capo, A Pennacchio… - Sensors, 2021 - mdpi.com
The purpose of this work is to provide an exhaustive overview of the emerging biosensor
technologies for the detection of analytes of interest for food, environment, security, and …

Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging

K Mishra, JP Fuenzalida-Werner, F Pennacchietti… - Nature …, 2022 - nature.com
Reversibly photo-switchable proteins are essential for many super-resolution fluorescence
microscopic and optoacoustic imaging methods. However, they have yet to be used as …

Genetically encoded, pH-sensitive mTFP1 biosensor for probing lysosomal pH

MY Chin, AR Patwardhan, KH Ang, AL Wang… - ACS …, 2021 - ACS Publications
Lysosomes are important sites for macromolecular degradation, defined by an acidic
lumenal pH of∼ 4.5. To better understand lysosomal pH, we designed a novel, genetically …

Visual pH sensors: from a chemical perspective to new bioengineered materials

L Di Costanzo, B Panunzi - Molecules, 2021 - mdpi.com
Many human activities and cellular functions depend upon precise pH values, and pH
monitoring is considered a fundamental task. Colorimetric and fluorescence sensors for pH …

Dual-reporting transcriptionally linked genetically encoded fluorescent indicators resolve the spatiotemporal coordination of cytosolic abscisic acid and second …

R Waadt, P Köster, Z Andrés, C Waadt… - Plant Cell, 2020 - academic.oup.com
Deciphering signal transduction processes is crucial for understanding how plants sense
and respond to environmental changes. Various chemical compounds function as central …

Wireless and battery-free platforms for collection of biosignals

T Stuart, L Cai, A Burton, P Gutruf - Biosensors and Bioelectronics, 2021 - Elsevier
Recent progress in biosensors have quantitively expanded current capabilities in
exploratory research tools, diagnostics and therapeutics. This rapid pace in sensor …

Digital models in biotechnology: towards multi-scale integration and implementation

FSF Hartmann, IA Udugama, GM Seibold… - Biotechnology …, 2022 - Elsevier
Industrial biotechnology encompasses a large area of multi-scale and multi-disciplinary
research activities. With the recent megatrend of digitalization sweeping across all …

[HTML][HTML] Intracellular pH–Advantages and pitfalls of surface-enhanced Raman scattering and fluorescence microscopy–A review

A Jaworska, K Malek, A Kudelski - … Acta Part A: Molecular and Biomolecular …, 2021 - Elsevier
The value of pH in various parts of protoplasm can affect nearly all aspects of cell functions.
Therefore, the determination of intracellular acid-base features is required in many areas of …

Bioluminescence color-tuning firefly luciferases: engineering and prospects for real-time intracellular pH imaging and heavy metal biosensing

VR Viviani, GF Pelentir, VR Bevilaqua - Biosensors, 2022 - mdpi.com
Firefly luciferases catalyze the efficient production of yellow-green light under normal
physiological conditions, having been extensively used for bioanalytical purposes for over 5 …