[HTML][HTML] Zebrafish as an animal model for biomedical research
Zebrafish have several advantages compared to other vertebrate models used in modeling
human diseases, particularly for large-scale genetic mutant and therapeutic compound …
human diseases, particularly for large-scale genetic mutant and therapeutic compound …
[HTML][HTML] Consensus guidelines for the use and interpretation of angiogenesis assays
The formation of new blood vessels, or angiogenesis, is a complex process that plays
important roles in growth and development, tissue and organ regeneration, as well as …
important roles in growth and development, tissue and organ regeneration, as well as …
[HTML][HTML] Genome-editing technologies for gene and cell therapy
ML Maeder, CA Gersbach - Molecular therapy, 2016 - cell.com
Gene therapy has historically been defined as the addition of new genes to human cells.
However, the recent advent of genome-editing technologies has enabled a new paradigm in …
However, the recent advent of genome-editing technologies has enabled a new paradigm in …
[HTML][HTML] ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …
comprise a powerful class of tools that are redefining the boundaries of biological research …
[HTML][HTML] Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish
The widespread availability of programmable site-specific nucleases now enables targeted
gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate …
gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate …
A guide to genome engineering with programmable nucleases
H Kim, JS Kim - Nature Reviews Genetics, 2014 - nature.com
Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
V Pattanayak, S Lin, JP Guilinger, E Ma… - Nature …, 2013 - nature.com
The RNA-programmable Cas9 endonuclease cleaves double-stranded DNA at sites
complementary to a 20-base-pair guide RNA. The Cas9 system has been used to modify …
complementary to a 20-base-pair guide RNA. The Cas9 system has been used to modify …
[HTML][HTML] Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs
The CRISPR/Cas9 system has been implemented in a variety of model organisms to
mediate site-directed mutagenesis. A wide range of mutation rates has been reported, but at …
mediate site-directed mutagenesis. A wide range of mutation rates has been reported, but at …
TALENs: a widely applicable technology for targeted genome editing
JK Joung, JD Sander - Nature reviews Molecular cell biology, 2013 - nature.com
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of
cell types and organisms. The newly-developed transcription activator-like effector …
cell types and organisms. The newly-developed transcription activator-like effector …
Review of CRISPR/Cas9 sgRNA design tools
The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …