Photoinduced chemistry in fluorescent proteins: curse or blessing?

A Acharya, AM Bogdanov, BL Grigorenko… - Chemical …, 2017 - ACS Publications
Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Current trends in protein engineering: updates and progress

R Sinha, P Shukla - Current Protein and Peptide Science, 2019 - ingentaconnect.com
Proteins are one of the most important and resourceful biomolecules that find applications in
health, industry, medicine, research, and biotechnology. Given its tremendous relevance …

Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography

N Coquelle, M Sliwa, J Woodhouse, G Schirò… - Nature Chemistry, 2018 - nature.com
Chromophores absorb light in photosensitive proteins and thereby initiate fundamental
biological processes such as photosynthesis, vision and biofluorescence. An important goal …

Laboratory evolution of protein conformational dynamics

EC Campbell, GJ Correy, PD Mabbitt, AM Buckle… - Current Opinion in …, 2018 - Elsevier
Highlights•Laboratory directed evolution and ancestral reconstruction can facilitate study of
protein structural dynamics.•Advances in biophysical and computational techniques facilitate …

Computational challenges in modeling of representative bioimaging proteins: GFP-like proteins, flavoproteins, and phytochromes

AV Nemukhin, BL Grigorenko… - The Journal of …, 2019 - ACS Publications
Remarkable success in engineering novel efficient biomarkers based on fluorescent and
photoactive proteins provokes a question of whether computational modeling of their …

Optimizing the fluorescent protein toolbox and its use

S Duwé, P Dedecker - Current opinion in biotechnology, 2019 - Elsevier
Highlights•Fluorescent proteins continue to be essential tools in life sciences.•
Phototransformable FPs and biosensors facilitate advanced imaging experiments.• …

Rationally designed influenza virus vaccines that are antigenically stable during growth in eggs

AT Harding, BE Heaton, RE Dumm, NS Heaton - MBio, 2017 - Am Soc Microbiol
Influenza virus vaccine production is currently limited by the ability to grow circulating human
strains in chicken eggs or in cell culture. To facilitate cost-effective growth, vaccine strains …

Homopolymeric Protein Phosphors: Overpassing the Stability Frontier of Deep‐Red Bio‐Hybrid Light‐Emitting Diodes

S Ferrara, JP Fernandéz‐Blázquez… - Advanced Functional …, 2023 - Wiley Online Library
Although protein‐polymer phosphors are an emerging photon‐management filter concept
for hybrid light‐emitting diodes, deep‐red‐emitting devices based on archetypal fluorescent …

New imaging probes to track cell fate: reporter genes in stem cell research

P Jurgielewicz, S Harmsen, E Wei… - Cellular and Molecular …, 2017 - Springer
Cell fate is a concept used to describe the differentiation and development of a cell in its
organismal context over time. It is important in the field of regenerative medicine, where stem …

Monomerization of far-red fluorescent proteins

TM Wannier, SK Gillespie, N Hutchins… - Proceedings of the …, 2018 - National Acad Sciences
Anthozoa-class red fluorescent proteins (RFPs) are frequently used as biological markers,
with far-red (λem∼ 600–700 nm) emitting variants sought for whole-animal imaging …