Gene delivery using nonviral approaches has been extensively studied as a basic tool for
intracellular gene transfer and gene therapy. In the past, the primary focus has been on …

Efficient and safe methods for delivering genetic materials into cells must be developed
before the clinical potential of gene therapy can be fully realized. Recently, hydrodynamic …

Over the last 5 years, physical methods of plasmid delivery have revolutionized the
efficiency of nonviral gene transfer, in some cases reaching the efficiencies of viral vectors …

Background Alpha-1 antitrypsin deficiency (AATD) is a rare hereditary condition that leads to
decreased circulating alpha-1 antitrypsin (AAT) levels, significantly increasing the risk of …

We have reported that a rapid tail vein injection of a large volume of plasmid DNA solution
into a mouse results in high level of transgene expression in the liver. Gene transfer …

Promising improvements in the field of transcript therapeutics have clearly enhanced the
potential of mRNA as a new pillar for protein replacement therapies. Synthetic mRNAs are …

Over the last few years, hydrodynamic tail vein delivery has established itself as a simple,
yet very effective method for gene transfer into small rodents. Hydrodynamic delivery of …

The work demonstrated the development of thermally cross-linked superparamagnetic
nanomaterial which possessed polyethylene glycol moiety and covalently linked branched …

Minicircle DNA vectors devoid of plasmid bacterial backbone,(BB) DNAs, are
transcriptionally persistent, whereas their parent plasmid counterparts are silenced in the …

The impact of hydrodynamic injection on liver structure was evaluated in mice using various
microscopic techniques. Upon hydrodynamic injection of approximately 9% of body weight …