Bacteria are simple and cost effective hosts for producing recombinant proteins. However,
their physiological features may limit their use for obtaining in native form proteins of some …

The advent of recombinant DNA technology has revolutionized the strategies for protein
production. Due to the well-characterized genome and a variety of mature tools available for …

Despite the fundamental importance of E. coli in the manufacture of a wide range of
biotechnological and biomedical products, extensive process and/or target optimisation is …

We demonstrate that small engineered single-chain binding proteins based on the lipocalin
scaffold, so-called Anticalins, can be functionally displayed on the Gram-negative bacterial …

The biogenesis of periplasmic and outer membrane proteins (OMPs) in Escherichia coli is
assisted by a variety of processes that help with their folding and transport to their final …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Abstract Comparison of different membrane anchor motifs for the surface display of a protein
of interest (passenger) is crucial for achieving the best possible performance. However …

The “seventeen kilodalton protein”(Skp) is a predominant periplasmic chaperone of
Escherichia coli, which is involved in the biogenesis of abundant outer membrane proteins …

Background Cell display technologies (eg bacterial display) are attractive in directed
evolution as they provide the option to use flow-cytometric cell sorting for selection from …

Experimental protein engineering and computational protein design are broad but
complementary strategies for developing proteins with altered or novel structural properties …