The microbiology, epidemiology, diagnostics, and treatment of infective endocarditis (IE)
have changed significantly since the Duke Criteria were published in 1994 and modified in …

Background Metagenomic next-generation sequencing (mNGS) for pathogen detection is
becoming increasingly available as a method to identify pathogens in cases of suspected …

Microbial cell-free DNA (mcfDNA) sequencing is an emerging infectious disease diagnostic
tool which enables unbiased pathogen detection and quantification from plasma. The Karius …

Background Plasma cell-free DNA Next-Generation Sequencing has been used as a non-
invasive and comprehensive method for the etiological diagnosis of infectious diseases …

Initial microbiologic diagnosis of infective endocarditis (IE) relies on blood cultures and
Bartonella and Coxiella burnetii serology. Small case series and one prospective study have …

Infective endocarditis (IE) is a life-threatening microbial infection of native and prosthetic
heart valves, endocardial surface, and/or indwelling cardiac device. Prevalence of IE is …

Blood cultures have been the gold standard for identifying pathogens in infective
endocarditis (IE). Blood culture-negative endocarditis (BCNE), however, occurs in 40% or …

Blood culture-negative infective endocarditis (BCNE) is a challenging condition associated
with significant morbidity and mortality. This review discusses the epidemiology …

Infective endocarditis is a potentially fatal condition, and identifying the pathogen is crucial to
optimizing antibiotic treatment. While a blood culture takes time and may yield negative …

Plasma cell-free metagenomic next-generation sequencing (cf-mNGS) is a non-invasive
method that may be able to identify thousands of pathogens through a hypothesis-free …