Structures of the nitrogenase complex prepared under catalytic turnover conditions

HL Rutledge, BD Cook, HPM Nguyen, MA Herzik Jr… - Science, 2022 - science.org
The enzyme nitrogenase couples adenosine triphosphate (ATP) hydrolysis to the
multielectron reduction of atmospheric dinitrogen into ammonia. Despite extensive research …

[HTML][HTML] Emerging procedures and solvents in biological sample pre-treatment

V Greco, A Giuffrida, M Locatelli, F Savini… - Advances in Sample …, 2023 - Elsevier
The treatment of biological samples, especially from complex matrices, has consistently
challenged analytical operators. The classic problems to be faced for any analysis …

Scaling up cryo-EM for biology and chemistry: The journey from niche technology to mainstream method

MJ de la Cruz, ET Eng - Structure, 2023 - cell.com
Cryoelectron microscopy (cryo-EM) methods have made meaningful contributions in a wide
variety of scientific research fields. In structural biology, cryo-EM routinely elucidates …

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

B Basanta, W Chen, DE Pride, GC Lander - JoVE (Journal of Visualized …, 2023 - jove.com
Cryogenic electron microscopy (cryoEM) has emerged as a powerful technique for probing
the atomic structure of macromolecular complexes. Sample preparation for cryoEM requires …

Single-particle cryo-EM data collection with stage tilt using leginon

S Aiyer, TS Strutzenberg, ME Bowman… - Journal of …, 2022 - pmc.ncbi.nlm.nih.gov
Single-particle analysis (SPA) by cryo-electron microscopy (cryo-EM) is now a mainstream
technique for high-resolution structural biology. Structure determination by SPA relies upon …

Magnetodynamics of few-nanoparticle chains

TQ Bui, SD Oberdick, FM Abel, MJ Donahue… - arXiv preprint arXiv …, 2024 - arxiv.org
In recent years, there has been increasing interest in the understanding and application of
nanoparticle assemblies driven by external fields. Although these systems can exhibit …

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

B Basanta, W Chen, DE Pride… - JoVE (Journal of Visualized …, 2023 - app.jove.com
Cryogenic electron microscopy (cryoEM) has emerged as a powerful technique for probing
the atomic structure of macromolecular complexes. Sample preparation for cryoEM requires …

[HTML][HTML] Tuning ice thickness using the chameleon for high-quality cryoEM data collection

KL McGuire, BD Cook, SM Narehood, MA Herzik Jr - bioRxiv, 2024 - ncbi.nlm.nih.gov
Advances in single-particle cryogenic electron microscopy (cryoEM) now allow for routine
structure determination of well-behaved biological specimens to high-resolution. Despite …

Recent Advances and Best Practices for Cryo-EM Analysis for Protein Structure Determination

J Bergeron, C Rapisarda - JoVE (Journal of Visualized Experiments), 2023 - jove.com
ARTICLES DISCUSSED: de Martin Garrido, N., Ramlaul, K., Aylett, CHS Preparation of
sample support films in transmission electron microscopy using a support floatation block …

[图书][B] Redox Dynamics of Nitrogenase

HL Rutledge - 2022 - search.proquest.com
Nitrogen is an essential component of many biomolecules such as DNA and proteins.
Biological nitrogen fixation is carried out by the enzyme nitrogenase. This multi-electron …