Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications

J Sankaran, T Wohland - Communications Biology, 2023 - nature.com
Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the
quantitative measurement of biomolecular dynamics and interactions. Improvements in …

Fluorescence Microscopy: a statistics-optics perspective

M Fazel, KS Grussmayer, B Ferdman, A Radenovic… - Reviews of Modern …, 2024 - APS
Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

Resolution enhancement with deblurring by pixel reassignment

B Zhao, J Mertz - Advanced photonics, 2023 - spiedigitallibrary.org
Improving the spatial resolution of a fluorescence microscope has been an ongoing
challenge in the imaging community. To address this challenge, a variety of approaches …

Visualizing proteins by expansion microscopy

AH Shaib, AA Chouaib, R Chowdhury, D Mihaylov… - bioRxiv, 2022 - biorxiv.org
Fluorescence imaging is one of the most versatile and widely-used tools in biology.
Although techniques to overcome the diffraction barrier were introduced more than two …

The Cby3/ciBAR1 complex positions the annulus along the sperm flagellum during spermiogenesis

M Hoque, FQ Li, WD Weber, JJ Chen, EN Kim… - Journal of Cell …, 2024 - rupress.org
Proper compartmentalization of the sperm flagellum is essential for fertility. The annulus is a
septin-based ring that demarcates the midpiece (MP) and the principal piece (PP). It is …

Fluorescence radial fluctuation enables two-photon super-resolution microscopy

M Tsutsumi, T Takahashi, K Kobayashi… - Frontiers in Cellular …, 2023 - frontiersin.org
Despite recent improvements in microscopy, it is still difficult to apply super-resolution
microscopy for deep imaging due to the deterioration of light convergence properties in thick …

Optical super-resolution imaging: A review and perspective

K Aflalo, P Gao, V Trivedi, A Sanjeev… - Optics and Lasers in …, 2024 - Elsevier
In this comprehensive review, we delve into super-resolution optical imaging techniques
and their diverse applications. Our primary focus is on linear optics super-resolution …

Improving STED microscopy with SUPPOSe: enhancing resolution from a single-image

M Toscani, AM Lacapmesure - Methods and Applications in …, 2024 - iopscience.iop.org
Here we apply the SUPPOSe algorithm on images acquired using Stimulated Emission
Depletion (STED) microscopy with the aim of improving the resolution limit achieved. We …

Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile

J Acosta-Cárdenas, LF Jiménez-García… - International Journal of …, 2024 - mdpi.com
Nuclear speckles are compartments enriched in splicing factors present in the nucleoplasm
of eucaryote cells. Speckles have been studied in mammalian culture and tissue cells, as …

Application of light sensors based on reinforcement learning in martial arts action optimization and image defect detection

PL Yan, YH Zhang - Optical and Quantum Electronics, 2024 - Springer
Wushu has unique movement requirements and techniques, but the traditional methods of
optimizing wushu have some problems, such as unstable effect and difficult quantitative …