Single-cell and spatial transcriptomics: deciphering brain complexity in health and disease
In the past decade, single-cell technologies have proliferated and improved from their
technically challenging beginnings to become common laboratory methods capable of …
technically challenging beginnings to become common laboratory methods capable of …
Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …
you to profile the whole transcriptome of a large number of individual cells. However, the …
A guide to ComBat harmonization of imaging biomarkers in multicenter studies
The impact of PET image acquisition and reconstruction parameters on SUV measurements
or radiomic feature values is widely documented. This scanner effect is detrimental to the …
or radiomic feature values is widely documented. This scanner effect is detrimental to the …
A living biobank of breast cancer organoids captures disease heterogeneity
Breast cancer (BC) comprises multiple distinct subtypes that differ genetically,
pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of …
pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of …
[HTML][HTML] Data-analysis strategies for image-based cell profiling
Image-based cell profiling is a high-throughput strategy for the quantification of phenotypic
differences among a variety of cell populations. It paves the way to studying biological …
differences among a variety of cell populations. It paves the way to studying biological …
Spatial genomic heterogeneity in multiple myeloma revealed by multi-region sequencing
In multiple myeloma malignant plasma cells expand within the bone marrow. Since this site
is well-perfused, a rapid dissemination of “fitter” clones may be anticipated. However, an …
is well-perfused, a rapid dissemination of “fitter” clones may be anticipated. However, an …
[HTML][HTML] Induced-pluripotent-stem-cell-derived primitive macrophages provide a platform for modeling tissue-resident macrophage differentiation and function
Tissue macrophages arise during embryogenesis from yolk-sac (YS) progenitors that give
rise to primitive YS macrophages. Until recently, it has been impossible to isolate or derive …
rise to primitive YS macrophages. Until recently, it has been impossible to isolate or derive …
[HTML][HTML] Data harmonisation for information fusion in digital healthcare: A state-of-the-art systematic review, meta-analysis and future research directions
Removing the bias and variance of multicentre data has always been a challenge in large
scale digital healthcare studies, which requires the ability to integrate clinical features …
scale digital healthcare studies, which requires the ability to integrate clinical features …
[HTML][HTML] Identifying cell populations with scRNASeq
TS Andrews, M Hemberg - Molecular aspects of medicine, 2018 - Elsevier
Single-cell RNASeq (scRNASeq) has emerged as a powerful method for quantifying the
transcriptome of individual cells. However, the data from scRNASeq experiments is often …
transcriptome of individual cells. However, the data from scRNASeq experiments is often …
Performance comparison of modified ComBat for harmonization of radiomic features for multicenter studies
Multicenter studies are needed to demonstrate the clinical potential value of radiomics as a
prognostic tool. However, variability in scanner models, acquisition protocols and …
prognostic tool. However, variability in scanner models, acquisition protocols and …