Burns are a prevalent and burdensome critical care problem. The priorities of specialized
facilities focus on stabilizing the patient, preventing infection, and optimizing functional …

Cultures of growth-arrested feeder cells have been used for years to promote cell
proliferation, particularly with low-density inocula. Basically, feeder cells consist in a layer of …

The therapy of burns is a challenging clinical issue. Burns are long-term injuries, and
numerous patients suffer from chronic pain. Burn treatment includes management, infection …

Since one of the main challenges in treating acute burn injuries is preventing infection, early
excising of the eschar and covering of the wound becomes critical. Non-viable tissue is …

Current limitations to primary cell expansion led us to test whether airway epithelial cells
derived from healthy children and those with asthma and cystic fibrosis (CF), co-cultured …

Split-thickness skin autografts (AGs) are the standard surgical treatment for severe burn
injuries. However, the treatment of patients with substantial skin loss is limited by the …

Autologous epidermal cultures restore a functional epidermis on burned patients.
Transgenic epidermal grafts do so also in genetic skin diseases such as Junctional …

Long-term primary culture of mammalian cells has been always difficult due to unavoidable
senescence. Conventional methods for generating immortalized cell lines usually require …

Laminin 332-deficient junctional epidermolysis bullosa (JEB) is a severe genetic skin
disease. JEB is marked by epidermal stem cell depletion, the origin of which is unknown. We …

Each day, about 2000 US workers have a job-related eye injury requiring medical treatment.
Corneal diseases are the fifth cause of blindness worldwide. Most of these diseases can be …