Within mammalian systems, there exists enormous opportunity to use synthetic gene circuits
to enhance phenotype-based drug discovery, to map the molecular origins of disease, and …

Phase separation underlies the formation of biomolecular condensates. We hypothesize the
cellular processes that occur within condensates shape their structural features. We use the …

Transcription induces a wave of DNA supercoiling, altering the binding affinity of RNA
polymerases and reshaping the biochemical landscape of gene regulation. As supercoiling …

Many cardiovascular diseases originate from growth retardation, inflammation, and
malnutrition during early postnatal development. The nature of this phenomenon is not …

Augmenting cells with novel, genetically encoded functions will support therapies that
expand beyond natural capacity for immune surveillance and tissue regeneration. However …

Single cell biology has the potential to elucidate many critical biological processes and
diseases, from development and regeneration to cancer. Single cell analyses are …

Gene syntax—the order and arrangement of genes and their regulatory elements—shapes
the dynamic coordination of both natural and synthetic gene circuits. Transcription at one …

While transcription factors (TFs) provide essential cues for directing and redirecting cell fate,
TFs alone are insufficient to drive cells to adopt alternative fates. Rather, transcription factors …