Repeat-unit elongations to produce bacterial complex long polysaccharide chains, an O-antigen perspective
The O-antigen, a long polysaccharide that constitutes the distal part of the outer membrane-
anchored lipopolysaccharide, is one of the critical components in the protective outer …
anchored lipopolysaccharide, is one of the critical components in the protective outer …
Progress in our understanding of Wzx flippase for translocation of bacterial membrane lipid-linked oligosaccharide
Y Hong, MA Liu, PR Reeves - Journal of Bacteriology, 2018 - Am Soc Microbiol
Translocation of lipid-linked oligosaccharides is a common theme across prokaryotes and
eukaryotes. For bacteria, such activity is used in cell wall construction, polysaccharide …
eukaryotes. For bacteria, such activity is used in cell wall construction, polysaccharide …
Decoding capsule synthesis in Streptococcus pneumoniae
T Su, R Nakamoto, YY Chun, WZ Chua… - FEMS Microbiology …, 2021 - academic.oup.com
Streptococcus pneumoniae synthesizes> 100 types of capsular polysaccharides (CPSs).
While the diversity of the enzymes and transporters involved is enormous, it is not limitless …
While the diversity of the enzymes and transporters involved is enormous, it is not limitless …
[HTML][HTML] The role of O-antigen in P1 transduction of Shigella flexneri and Escherichia coli with its alternative S'tail fibre
Enterobacteria phage P1 expresses two types of tail fibre, S and S'. Despite the wide usage
of phage P1 for transduction, the host range and the receptor for its alternative S'tail fibre …
of phage P1 for transduction, the host range and the receptor for its alternative S'tail fibre …
Identification of the Shigella flexneri Wzy Domain Modulating WzzpHS-2 Interaction and Detection of the Wzy/Wzz/Oag Complex
A Ascari, ENH Tran, BA Eijkelkamp… - Journal of …, 2022 - Am Soc Microbiol
Shigella flexneri implements the Wzy-dependent pathway to biosynthesize the O antigen
(Oag) component of its surface lipopolysaccharide. The inner membrane polymerase WzySF …
(Oag) component of its surface lipopolysaccharide. The inner membrane polymerase WzySF …
Detection of Wzy/Wzz interaction in Shigella flexneri
The O antigen (Oag) component of Shigella flexneri lipopolysaccharide (LPS) is important
for virulence and a protective antigen. It is synthesized by the Wzy-dependent mechanism …
for virulence and a protective antigen. It is synthesized by the Wzy-dependent mechanism …
Polysaccharide copolymerase WzzB/WzzE chimeras reveal that the transmembrane 2 region of WzzB is important for interaction with WzyB
The ability of bacteria to synthesize complex polysaccharide chains at a controlled number
of repeating units has wide implications for a range of biological activities that include …
of repeating units has wide implications for a range of biological activities that include …
Determining glycosyltransferase functional order via lethality due to accumulated O-antigen intermediates, exemplified with Shigella flexneri O-antigen biosynthesis
The O antigen (OAg) polysaccharide is one of the most diverse surface molecules of Gram-
negative bacterial pathogens. The structural classification of OAg, based on serological …
negative bacterial pathogens. The structural classification of OAg, based on serological …
Three Wzy polymerases are specific for particular forms of an internal linkage in otherwise identical O units
Y Hong, VA Morcilla, MA Liu, ELM Russell… - …, 2015 - microbiologyresearch.org
The Wzx/Wzy-dependent pathway is the predominant pathway for O-antigen production in
Gram-negative bacteria. The O-antigen repeat unit (O unit) is an oligosaccharide that is …
Gram-negative bacteria. The O-antigen repeat unit (O unit) is an oligosaccharide that is …
[HTML][HTML] Detection of a disulphide bond and conformational changes in Shigella flexneri Wzy, and the role of cysteine residues in polymerase activity
A Ascari, ENH Tran, BA Eijkelkamp… - Biochimica et Biophysica …, 2022 - Elsevier
Shigella flexneri utilises the Wzy-dependent pathway for the production of a plethora of
complex polysaccharides, including the lipopolysaccharide O-antigen (Oag) component …
complex polysaccharides, including the lipopolysaccharide O-antigen (Oag) component …