Role of cholesterol in the function and organization of G-protein coupled receptors

TJ Pucadyil, A Chattopadhyay - Progress in lipid research, 2006 - Elsevier
Cholesterol is an essential component of eukaryotic membranes and plays a crucial role in
membrane organization, dynamics and function. The modulatory role of cholesterol in the …

The Serotonin 1A A Receptor: A Representative Member of the Serotonin Receptor Family

TJ Pucadyil, S Kalipatnapu… - Cellular and molecular …, 2005 - Springer
Serotonin is an intrinsically fluorescent biogenic amine that acts as a neurotransmitter and is
found in a wide variety of sites in the central and peripheral nervous system. Serotonergic …

[HTML][HTML] Cholesterol modulates ligand binding and G-protein coupling to serotonin1A receptors from bovine hippocampus

TJ Pucadyil, A Chattopadhyay - Biochimica et Biophysica Acta (BBA) …, 2004 - Elsevier
The serotonin1A (5-HT1A) receptor is an important member of the superfamily of seven-
transmembrane domain G-protein-coupled receptors. We have examined the modulatory …

Membrane protein solubilization: recent advances and challenges in solubilization of serotonin1A receptors

S Kalipatnapu, A Chattopadhyay - IUBMB life, 2005 - Wiley Online Library
Solubilization of integral membrane proteins is a process in which the proteins and lipids
that are held together in native membranes are suitably dissociated in a buffered detergent …

[18F] MPPF as a tool for the in vivo imaging of 5-HT1A receptors in animal and human brain

N Aznavour, L Zimmer - Neuropharmacology, 2007 - Elsevier
Serotonin (5-hydroxytryptamine, 5-HT) and its various receptors are involved in numerous
CNS functions and psychiatric disorders. 5-HT1A, the best-characterized subtype of …

Membrane Organization and Function of the Serotonin1A Receptor

S Kalipatnapu, A Chattopadhyay - Cellular and Molecular Neurobiology, 2007 - Springer
The serotonin 1A receptor is a G-protein coupled receptor involved in several cognitive,
behavioral, and developmental functions. It binds the neurotransmitter serotonin and signals …

G-Protein-Dependent Cell Surface Dynamics of the Human Serotonin1A Receptor Tagged to Yellow Fluorescent Protein

TJ Pucadyil, S Kalipatnapu, KG Harikumar… - Biochemistry, 2004 - ACS Publications
Serotonergic signaling appears to play a key role in the generation and modulation of
various cognitive, behavioral, and developmental processes. The serotonin1A receptor is an …

Role of cholesterol in ligand binding and G-protein coupling of serotonin1A receptors solubilized from bovine hippocampus

A Chattopadhyay, M Jafurulla, S Kalipatnapu… - Biochemical and …, 2005 - Elsevier
The serotonin1A (5-HT1A) receptor is an important member of the superfamily of seven
transmembrane domain G-protein-coupled receptors. We report here that solubilization of …

Ligand Binding Characteristics of the Human Serotonin1A Receptor Heterologously Expressed in CHO Cells

S Kalipatnapu, TJ Pucadyil, KG Harikumar… - Bioscience …, 2004 - portlandpress.com
The serotonin1 A (5-HT1 A) receptors are important members of the superfamily of seven
transmembrane domain G-protein coupled receptors. They appear to be involved in various …

Impact of purification conditions and history on A2A adenosine receptor activity: The role of CHAPS and lipids

AN Naranjo, PM McNeely, J Katsaras… - Protein expression and …, 2016 - Elsevier
The adenosine A 2A receptor (A 2A R) is a much-studied class AG protein-coupled receptor
(GPCR). For biophysical studies, A 2A R is commonly purified in a detergent mixture of …