Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9
system, a groundbreaking innovation in genetic engineering, has revolutionized our …

N1-methyladenosine (m1A) modification is an epigenetic change that occurs on RNA
molecules, regulated by a suite of enzymes including methyltransferases (writers) …

Gene-edited animals are crucial for addressing fundamental questions in biology and
medicine and hold promise for practical applications. In light of the rapid advancement of …

Forests worldwide are under increasing pressure from climate change and emerging
diseases, threatening their vital ecological and economic roles. Traditional breeding …

Neurodegenerative disease characterized by the progressive damage of the nervous
system, and neuropathies caused by the neuronal injury are both led to substantial …

Identifying off-target effects of genome editing with Tracking-seq | Nature Reviews Genetics
Skip to main content Thank you for visiting nature.com. You are using a browser version with …

The paired nickases approach, which utilizes clustered regularly interspaced short
palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) nickase and dual guide …

The ability to recognize antigen epitope is crucial for generating an effective immune
response. By engineering these epitopes, researchers can reduce on-target/off-tumor …

Programmable epigenome editors modify gene expression in mammalian cells by altering
the local chromatin environment at target loci without inducing DNA breaks. However, the …

CRISPR nuclease-mediated gene knock-out is limited by suboptimal sgRNAs, inaccessible
target sites, and silent mutations. Here, we present a Cas12a-based system that targets …