Present and future of culturing bacteria

J Overmann, B Abt, J Sikorski - Annual review of microbiology, 2017 - annualreviews.org
The cultivation of bacteria is highly biased toward a few phylogenetic groups. Many of the
currently underexplored bacterial lineages likely have novel biosynthetic pathways and …

Cultivation of unculturable soil bacteria

VHT Pham, J Kim - Trends in biotechnology, 2012 - cell.com
Despite the abundance of bacterial species in soil, more than 99% of these species cannot
be cultured by traditional techniques. In addition, the less than 1% of bacteria that can be …

Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes

KS Ramirez, JM Craine, N Fierer - Global change biology, 2012 - Wiley Online Library
Ecosystems worldwide are receiving increasing amounts of reactive nitrogen (N) via
anthropogenic activities with the added N having potentially important impacts on …

The under-recognized dominance of Verrucomicrobia in soil bacterial communities

GT Bergmann, ST Bates, KG Eilers, CL Lauber… - Soil Biology and …, 2011 - Elsevier
Verrucomicrobia are ubiquitous in soil, but members of this bacterial phylum are thought to
be present at low frequency in soil, with few studies focusing specifically on verrucomicrobial …

Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium

M Derrien, EE Vaughan… - … of systematic and …, 2004 - microbiologyresearch.org
The diversity of mucin-degrading bacteria in the human intestine was investigated by
combining culture and 16S rRNA-dependent approaches. A dominant bacterium, strain …

Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity

P Hugenholtz, BM Goebel, NR Pace - Journal of bacteriology, 1998 - Am Soc Microbiol
Our perspective on microbial diversity has improved enormously over the past few decades.
In large part this has been due to molecular phylogenetic studies that objectively relate …

The uncultured microbial majority

MS Rappé, SJ Giovannoni - Annual Reviews in Microbiology, 2003 - annualreviews.org
▪ Abstract Since the delineation of 12 bacterial phyla by comparative phylogenetic analyses
of 16S ribosomal RNA in 1987 knowledge of microbial diversity has expanded dramatically …

Biosignatures on Mars: what, where, and how? Implications for the search for martian life

F Westall, F Foucher, N Bost, M Bertrand, D Loizeau… - Astrobiology, 2015 - liebertpub.com
The search for traces of life is one of the principal objectives of Mars exploration. Central to
this objective is the concept of habitability, the set of conditions that allows the appearance …

The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set

H Daims, A Brühl, R Amann, KH Schleifer… - Systematic and applied …, 1999 - Elsevier
In situ hybridization with rRNA-targeted oligonucleotide probes has become a widely
applied tool for direct analysis of microbial population structures of complex natural and …

Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria, Actinobacteria, Proteobacteria, and …

PH Janssen, PS Yates, BE Grinton… - Applied and …, 2002 - Am Soc Microbiol
The culturability of bacteria in the bulk soil of an Australian pasture was investigated by
using nutrient broth at 1/100 of its normal concentration (dilute nutrient broth [DNB]) as the …