Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …

Modern research in the life sciences is unthinkable without computational methods for
extracting, quantifying and visualising information derived from microscopy imaging data of …

Single-cell omics technologies have enabled the creation of comprehensive cell atlases
across tissues and species and delivered key insights into the biological mechanisms …

Spatially resolved omics technologies are transforming our understanding of biological
tissues. However, the handling of uni-and multimodal spatial omics datasets remains a …

A growing community is constructing a next-generation file format (NGFF) for bioimaging to
overcome problems of scalability and heterogeneity. Organized by the Open Microscopy …

Single-cell and spatial transcrip-tomics illuminate complementary features of tissues.
Computational integration can synergize these technologies to resolve cell types and …

Background Computational pathology has seen rapid growth in recent years, driven by
advanced deep-learning algorithms. Due to the sheer size and complexity of multi-gigapixel …

Light sheet microscopy is a powerful technique for high-speed three-dimensional imaging of
subcellular dynamics and large biological specimens. However, it often generates datasets …

Light-sheet fluorescence microscopy (LSFM) enables fast, gentle and large-volume imaging,
making it an increasingly important tool for cell biological discovery. In both live cells and …

The imminent release of tissue atlases combining multichannel microscopy with single-cell
sequencing and other omics data from normal and diseased specimens creates an urgent …