The formation of microbial biofilms enables single planktonic cells to assume a multicellular
mode of growth. During dispersion, the final step of the biofilm life cycle, single cells egress …

Microbial communities enmeshed in a matrix of macromolecules, termed as biofilms, are the
natural setting of bacteria. Exopolysaccharide is a critical matrix component of biofilms …

Antimicrobial drug resistance is a growing threat to global public health. Multidrug resistance
among the'ESKAPE'organisms—encompassing Enterococcus faecium, Staphylococcus …

Allelic exchange is an efficient method of bacterial genome engineering. This protocol
describes the use of this technique to make gene knockouts and knock-ins, as well as single …

Despite enormous progress in understanding the fundamentals of bacterial gene regulation,
our knowledge remains limited when compared with the number of bacterial genomes and …

The virulence of Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, is
regulated by many transcriptional factors (TFs) that control the expression of quorum …

Microorganisms in the natural environment typically live on or in close association with
surfaces and predominantly exist as biofilms, surface attached microbial communities …

Abstract Mycobacterium tuberculosis (MTB) infects 30% of all humans and kills someone
every 20–30 s. Here we report genome-wide binding for~ 80% of all predicted MTB …

The type VI secretion system (T6SS) is a weapon of bacterial warfare and host cell
subversion. The Gram-negative pathogen Pseudomonas aeruginosa has three T6SSs …

Genome architecture has proven to be critical in determining gene regulation across almost
all domains of life. While many of the key components and mechanisms of eukaryotic …