[HTML][HTML] Oxidative stress and assisted reproduction: a comprehensive review of its pathophysiological role and strategies for optimizing embryo culture environment

A Agarwal, I Maldonado Rosas, C Anagnostopoulou… - Antioxidants, 2022 - mdpi.com
Oxidative stress (OS) due to an imbalance between reactive oxygen species (ROS) and
antioxidants has been established as an important factor that can negatively affect the …

The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction

PL Wale, DK Gardner - Human reproduction update, 2016 - academic.oup.com
BACKGROUND Although laboratory procedures, along with culture media formulations,
have improved over the past two decades, the issue remains that human IVF is performed in …

Impact of the IVF laboratory environment on human preimplantation embryo phenotype

DK Gardner, RL Kelley - Journal of Developmental Origins of Health …, 2017 - cambridge.org
The phenotype of the human embryo conceived through in vitro fertilization (IVF), that is its
morphology, developmental kinetics, physiology and metabolism, can be affected by …

[HTML][HTML] Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential

JE Swain, D Carrell, A Cobo, M Meseguer, C Rubio… - Fertility and sterility, 2016 - Elsevier
With increased use of comprehensive chromosome screening (CCS), the question remains
as to why some practices do not experience the same high levels of clinical success after …

Environmental and epigenetic effects upon preimplantation embryo metabolism and development

RJ Chason, J Csokmay, JH Segars… - Trends in Endocrinology …, 2011 - cell.com
In vitro fertilization has provided a unique window into the metabolic processes that drive
embryonic growth and development from a fertilized ovum to a competent blastocyst. Post …

Biological pH buffers in IVF: help or hindrance to success

MA Will, NA Clark, JE Swain - Journal of assisted reproduction and …, 2011 - Springer
Purpose Minimizing environmental stress helps maintain cellular homeostasis and is a
crucial component in optimizing embryo development in vitro and resulting ART success …

Is there an optimal pH for culture media used in clinical IVF?

JE Swain - Human reproduction update, 2012 - academic.oup.com
BACKGROUND Reducing environmental stress imposed upon gametes and embryos in the
IVF laboratory is crucial in optimizing culture conditions and development. One …

Metabolic activity of human blastocysts correlates with their morphokinetics, morphological grade, KIDScore and artificial intelligence ranking

L Ferrick, YSL Lee, DK Gardner - Human Reproduction, 2020 - academic.oup.com
STUDY QUESTION Is there a relationship between blastocyst metabolism and biomarkers of
embryo viability? SUMMARY ANSWER Blastocysts with higher developmental potential and …

Long-term and transgenerational effects of in vitro culture on mouse embryos

A Calle, R Fernandez-Gonzalez, P Ramos-Ibeas… - Theriogenology, 2012 - Elsevier
The mouse is a convenient model to analyze the impact of in vitro culture (IVC) on the long-
term health and physiology of the offspring, and the possible inheritance of these altered …

Optimal human embryo culture

JE Swain - Seminars in reproductive medicine, 2015 - thieme-connect.com
A large contributor to success during in vitro fertilization (IVF) lies in the processes occurring
within the IVF laboratory. These processes make up the “culture system.” This system entails …