Coacervate droplets for synthetic cells
The design and construction of synthetic cells–human‐made microcompartments that mimic
features of living cells–have experienced a real boom in the past decade. While many efforts …
features of living cells–have experienced a real boom in the past decade. While many efforts …
Fundamental Aspects of Phase-Separated Biomolecular Condensates
Biomolecular condensates, formed through phase separation, are upending our
understanding in much of molecular, cell, and developmental biology. There is an urgent …
understanding in much of molecular, cell, and developmental biology. There is an urgent …
Co-transcriptional production of programmable RNA condensates and synthetic organelles
Condensation of RNA and proteins is central to cellular functions, and the ability to program
it would be valuable in synthetic biology and synthetic cell science. Here we introduce a …
it would be valuable in synthetic biology and synthetic cell science. Here we introduce a …
DNA droplets for intelligent and dynamical artificial cells: From the viewpoint of computation and non-equilibrium systems
M Takinoue - Interface Focus, 2023 - royalsocietypublishing.org
Living systems are molecular assemblies whose dynamics are maintained by non-
equilibrium chemical reactions. To date, artificial cells have been studied from such physical …
equilibrium chemical reactions. To date, artificial cells have been studied from such physical …
Temporally controlled multistep division of DNA droplets for dynamic artificial cells
T Maruyama, J Gong, M Takinoue - Nature Communications, 2024 - nature.com
Synthetic droplets mimicking bio-soft matter droplets formed via liquid-liquid phase
separation (LLPS) in living cells have recently been employed in nanobiotechnology for …
separation (LLPS) in living cells have recently been employed in nanobiotechnology for …
Nucleic acid liquids
GR Abraham, AS Chaderjian… - Reports on Progress …, 2024 - iopscience.iop.org
The confluence of recent discoveries of the roles of biomolecular liquids in living systems
and modern abilities to precisely synthesize and modify nucleic acids (NAs) has led to a …
and modern abilities to precisely synthesize and modify nucleic acids (NAs) has led to a …
Light-controlled growth of DNA organelles in synthetic cells
Living cells regulate many of their vital functions through dynamic, membraneless
compartments that phase separate (condense) in response to different types of stimuli. In …
compartments that phase separate (condense) in response to different types of stimuli. In …
A facile DNA coacervate platform for engineering wetting, engulfment, fusion and transient behavior
Biomolecular coacervates are emerging models to understand biological systems and
important building blocks for designer applications. DNA can be used to build up …
important building blocks for designer applications. DNA can be used to build up …
Protein recruitment to dynamic DNA-RNA host condensates
We describe the design and characterization of artificial nucleic acid condensates that are
engineered to recruit and locally concentrate proteins of interest in vitro. These condensates …
engineered to recruit and locally concentrate proteins of interest in vitro. These condensates …
Liquid DNA coacervates form porous capsular hydrogels via viscoelastic phase separation on microdroplet interface
Liquid–liquid phase separation (LLPS) droplets of biopolymers are known as functional
microdroplets in living cells and have recently been used to construct protocells and artificial …
microdroplets in living cells and have recently been used to construct protocells and artificial …