Developments and opportunities in continuous biopharmaceutical manufacturing
O Khanal, AM Lenhoff - MAbs, 2021 - Taylor & Francis
Today's biologics manufacturing practices incur high costs to the drug makers, which can
contribute to high prices for patients. Timely investment in the development and …
contribute to high prices for patients. Timely investment in the development and …
[HTML][HTML] Applications of bio-capacitance to cell culture manufacturing
This review focuses on why bio-capacitance has developed to become the standard online
method to estimate biomass in cell-based bio-manufacturing processes. There is now a …
method to estimate biomass in cell-based bio-manufacturing processes. There is now a …
Challenges in process control for continuous processing for production of monoclonal antibody products
Continuous processing has been garnering much interest lately for production of
biopharmaceutical products. Purported benefits include higher productivity (10-15X) …
biopharmaceutical products. Purported benefits include higher productivity (10-15X) …
Taking the pulse of bioprocesses: at-line and in-line monitoring of mammalian cell cultures
CF Shek, M Betenbaugh - Current Opinion in Biotechnology, 2021 - Elsevier
Highlights•Current advancements in at-line and in-line analytical tools have enabled the
refinement of existing tools.•Spectroscopic methods continue to be promising tools for …
refinement of existing tools.•Spectroscopic methods continue to be promising tools for …
Rapid at‐line early cell death quantification using capacitance spectroscopy
Cell death is one of the failure modes of mammalian cell culture. Apoptosis is a regulated
cell death process mainly observed in cell culture. Timely detection of apoptosis onset …
cell death process mainly observed in cell culture. Timely detection of apoptosis onset …
Multivariate data analysis of capacitance frequency scanning for online monitoring of viable cell concentrations in small-scale bioreactors
S Metze, S Blioch, J Matuszczyk, G Greller… - Analytical and …, 2020 - Springer
Viable cell concentration (VCC) is one of the most important process attributes during
mammalian cell cultivations. Current state-of-the-art measurements of VCC comprise offline …
mammalian cell cultivations. Current state-of-the-art measurements of VCC comprise offline …
[HTML][HTML] Capacitance sensors in cell-based bioprocesses: online monitoring of biomass and more
I Surowiec, J Scholz - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights●Biocapacitance has become an established method for online biomass
monitoring.●Spectroscopy approaches allow more than only biomass determination.● …
monitoring.●Spectroscopy approaches allow more than only biomass determination.● …
Robust control of fed-batch high-cell density cultures: a simulation-based assessment
High-cell density cultures (HCDC), prone to metabolic overflow, are typically operated in fed-
batch mode to maximize productivity. We developed a simulation-based procedure for …
batch mode to maximize productivity. We developed a simulation-based procedure for …
Cole‐Cole modeling of real‐time capacitance data for estimation of cell physiological properties in recombinant Escherichia coli cultivation
Real‐time estimation of physiological properties of the cell during recombinant protein
production would ensure enhanced process monitoring. In this study, we explored the …
production would ensure enhanced process monitoring. In this study, we explored the …
Capacitance spectroscopy enables real‐time monitoring of early cell death in mammalian cell culture
Abstract Background/Aims Previous work developed a quantitative model using capacitance
spectroscopy in an at‐line setup to predict the dying cell percentage measured from a flow …
spectroscopy in an at‐line setup to predict the dying cell percentage measured from a flow …