General introduction: recombinant protein production and purification of insoluble proteins

N Ferrer-Miralles, P Saccardo, JL Corchero… - … Proteins: Methods and …, 2015 - Springer
Proteins are synthesized in heterologous systems because of the impossibility to obtain
satisfactory yields from natural sources. The production of soluble and functional …

Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications

S Mital, G Christie, D Dikicioglu - Microbial cell factories, 2021 - Springer
Recombinant enzyme expression in Escherichia coli is one of the most popular methods to
produce bulk concentrations of protein product. However, this method is often limited by the …

Yeast hosts for the production of recombinant laccases: a review

Z Antošová, H Sychrová - Molecular biotechnology, 2016 - Springer
Laccases are multi-copper oxidoreductases which catalyze the oxidation of a wide range of
substrates during the simultaneous reduction of oxygen to water. These enzymes, originally …

Efficient removal of sulfonamides and tetracyclines residues by the laccase-mediator system employing a novel laccase from Lysinibacillus fusiformis

B Ouyang, W Xu, W Zhang, C Guang, W Mu - Journal of Environmental …, 2022 - Elsevier
The laccase-mediator system (LMS) is an important solution to eliminate veterinary residues.
In this study, a novel laccase from Lysinibacillus fusiformis (Lyfu-Lac) showed promising …

The first acidobacterial laccase-like multicopper oxidase revealed by metagenomics shows high salt and thermo-tolerance

L Ausec, F Berini, C Casciello, MS Cretoiu… - Applied Microbiology …, 2017 - Springer
Metagenomics is a powerful tool that allows identifying enzymes with novel properties from
the unculturable component of microbiomes. However, thus far only a limited number of …

A simple strategy for extracellular production of CotA laccase in Escherichia coli and decolorization of simulated textile effluent by recombinant laccase

TN Wang, M Zhao - Applied Microbiology and Biotechnology, 2017 - Springer
Laccases are green oxidases with a number of potential industrial applications. In this study,
recombinant Bacillus subtilis CotA laccase was secreted by Escherichia coli via both the α …

Improving the Indigo Carmine Decolorization Ability of a Bacillus amyloliquefaciens Laccase by Site-Directed Mutagenesis

J Wang, L Lu, F Feng - Catalysts, 2017 - mdpi.com
Indigo carmine is a typical recalcitrant dye which is widely used in textile dyeing processes.
Laccases are versatile oxidases showing strong ability to eliminate hazardous dyes from …

An alkaline thermostable laccase from termite gut associated strain of Bacillus stratosphericus

Y Xiao, J Li, P Wu, N Ning, J Li, Y Shen… - International Journal of …, 2021 - Elsevier
Laccase, an important oxidoreductase, is widely distributed in various organisms. Termites
are known to decompose lignocellulose efficiently with the aid of gut microorganisms …

Recombinant protein production and purification of insoluble proteins

N Ferrer-Miralles, P Saccardo, JL Corchero… - … Proteins: Methods and …, 2022 - Springer
Proteins are synthesized in heterologous systems because of the impossibility to obtain
satisfactory yields from natural sources. The efficient production of soluble and functional …

Characterization of a thermo-alkali-stable laccase from Bacillus subtilis cjp3 and its application in dyes decolorization

W Qiao, J Chu, S Ding, X Song, L Yu - Journal of Environmental …, 2017 - Taylor & Francis
In this work, a novel bacterial strain exhibiting laccase activity was isolated from black liquor
and identified as Bacillus subtilis cjp3. The CotA-laccase gene was cloned from strain cjp3 …