Biomass is one of the most important variables in submerged-cultivation processes and, in
recent years, many different sensors have been introduced for on-line or in situ monitoring of …

Human basic fibroblast growth factor (hFGF‐2) was produced in high‐cell density cultures of
recombinant Escherichia coli using a temperature‐inducible expression system. The …

The need to operate bioreactors in an efficient manner is becoming more relevant in today's
environment of changing process technology and increasing market competition. Better …

Background The focus of this study is online estimation of biomass concentration in fed-
batch cultures. It describes a bioengineering software solution, which is explored for …

The purpose of this study is to introduce an improved Luedeking–Piret model that represents
a structurally simple biomass concentration approach. The developed routine provides …

Fed-batch production of recombinant fuculose-1-phosphate aldolase (FucA) by Escherichia
coli XL1 Blue MRF′(pTrcfuc) has been automated by using a simple feedback specific …

In high‐cell density cultures of Escherichia coli, the amount of ammonia required to maintain
a constant pH was an effective on‐line measure of biomass. When acetic acid formation …

The utilisation of tryptophan fluorescence as an indirect biomass measurement for the yeast
Pichia pastoris, an excellent host system for the production of heterologous proteins, is …

Estimation of biomass, substrate, and specific growth rate (μ) by two nonlinear observers
(nonlinear observer‐based estimator—NLOBE, asymptotic observer with second‐order …

Abstract [eng] In this work, the fields of informatics engineering and biotechnology are
combined in order to solve the fundamental problem of real-time recording of the most …