Probing native protein structures by chemical cross-linking, mass spectrometry, and bioinformatics

A Leitner, T Walzthoeni, A Kahraman, F Herzog… - Molecular & Cellular …, 2010 - ASBMB
Chemical cross-linking of reactive groups in native proteins and protein complexes in
combination with the identification of cross-linked sites by mass spectrometry has been in …

Cross-linking mass spectrometry for mapping protein complex topologies in situ

K Lee, FJ O'Reilly - Essays in Biochemistry, 2023 - portlandpress.com
Cross-linking mass spectrometry has become an established technology to provide
structural information on the topology and dynamics of protein complexes. Readily …

[HTML][HTML] The beginning of a beautiful friendship: cross-linking/mass spectrometry and modelling of proteins and multi-protein complexes

J Rappsilber - Journal of structural biology, 2011 - Elsevier
After more than a decade of method development, cross-linking in combination with mass
spectrometry and bioinformatics is finally coming of age. This technology now provides …

Expanding the chemical cross-linking toolbox by the use of multiple proteases and enrichment by size exclusion chromatography

A Leitner, R Reischl, T Walzthoeni, F Herzog… - Molecular & cellular …, 2012 - ASBMB
Chemical cross-linking in combination with mass spectrometric analysis offers the potential
to obtain low-resolution structural information from proteins and protein complexes …

Kojak: efficient analysis of chemically cross-linked protein complexes

MR Hoopmann, A Zelter, RS Johnson… - Journal of proteome …, 2015 - ACS Publications
Protein chemical cross-linking and mass spectrometry enable the analysis of protein–protein
interactions and protein topologies; however, complicated cross-linked peptide spectra …

PhoX: an IMAC-enrichable cross-linking reagent

B Steigenberger, RJ Pieters, AJR Heck… - ACS central …, 2019 - ACS Publications
Chemical cross-linking mass spectrometry is rapidly emerging as a prominent technique to
study protein structures. Structural information is obtained by covalently connecting peptides …

[HTML][HTML] Anatomy of a crosslinker

A Belsom, J Rappsilber - Current opinion in chemical biology, 2021 - Elsevier
Crosslinking mass spectrometry has become a core technology in structural biology and is
expanding its reach towards systems biology. Its appeal lies in a rapid workflow, high …

Combined sonodynamic and antimetabolite therapy for the improved treatment of pancreatic cancer using oxygen loaded microbubbles as a delivery vehicle

C McEwan, S Kamila, J Owen, H Nesbitt, B Callan… - Biomaterials, 2016 - Elsevier
In this manuscript we describe the preparation of an oxygen-loaded microbubble (O 2 MB)
platform for the targeted treatment of pancreatic cancer using both sonodynamic therapy …

Crosslinking combined with mass spectrometry for structural proteomics

EV Petrotchenko, CH Borchers - Mass spectrometry reviews, 2010 - Wiley Online Library
The method of crosslinking combined with mass spectrometry is being gradually accepted
as a technology enabling detailed structural information on proteins and protein complexes …

Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states

D Tan, Q Li, MJ Zhang, C Liu, C Ma, P Zhang, YH Ding… - Elife, 2016 - elifesciences.org
To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we
developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity …