Applications of CRISPR–Cas in agriculture and plant biotechnology

H Zhu, C Li, C Gao - Nature Reviews Molecular Cell Biology, 2020 - nature.com
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

Applications of CRISPR technologies in research and beyond

R Barrangou, JA Doudna - Nature biotechnology, 2016 - nature.com
Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …

Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs

EE Ellison, U Nagalakshmi, ME Gamo, P Huang… - Nature plants, 2020 - nature.com
An in planta gene editing approach was developed wherein Cas9 transgenic plants are
infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are …

Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture

T Li, J Hu, Y Sun, B Li, D Zhang, W Li, J Liu, D Li… - Molecular Plant, 2021 - cell.com
Genome editing provides novel strategies for improving plant traits but mostly relies on
conventional plant genetic transformation and regeneration procedures, which can be …

A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants

X Ma, Q Zhang, Q Zhu, W Liu, Y Chen, R Qiu, B Wang… - Molecular plant, 2015 - cell.com
CRISPR/Cas9 genome targeting systems have been applied to a variety of species.
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …

Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes

Q Liu, C Zhao, K Sun, Y Deng, Z Li - Molecular Plant, 2023 - cell.com
CRISPR/Cas genome-editing tools provide unprecedented opportunities for basic plant
biology research and crop breeding. However, the lack of robust delivery methods has …

CRISPR/Cas9-mediated viral interference in plants

Z Ali, A Abulfaraj, A Idris, S Ali, M Tashkandi… - Genome biology, 2015 - Springer
Abstract Background The CRISPR/Cas9 system provides bacteria and archaea with
molecular immunity against invading phages and conjugative plasmids. Recently …

[HTML][HTML] CRISPR/Cas9 platforms for genome editing in plants: developments and applications

X Ma, Q Zhu, Y Chen, YG Liu - Molecular plant, 2016 - cell.com
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9
(Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …

New frontiers in agriculture productivity: optimised microbial inoculants and in situ microbiome engineering

Z Qiu, E Egidi, H Liu, S Kaur, BK Singh - Biotechnology advances, 2019 - Elsevier
Increasing agricultural productivity is critical to feed the ever-growing human population.
Being linked intimately to plant health, growth and productivity, harnessing the plant …

Genome editing: targeting susceptibility genes for plant disease resistance

SSA Zaidi, MS Mukhtar, S Mansoor - Trends in Biotechnology, 2018 - cell.com
Plant pathogens pose a major threat to crop productivity. Typically, phytopathogens exploit
plants' susceptibility (S) genes to facilitate their proliferation. Disrupting these S genes may …