Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms. In the research arena, versatile CRISPR …
EE Ellison, U Nagalakshmi, ME Gamo, P Huang… - Nature plants, 2020 - nature.com
An in planta gene editing approach was developed wherein Cas9 transgenic plants are infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are …
T Li, J Hu, Y Sun, B Li, D Zhang, W Li, J Liu, D Li… - Molecular Plant, 2021 - cell.com
Genome editing provides novel strategies for improving plant traits but mostly relies on conventional plant genetic transformation and regeneration procedures, which can be …
X Ma, Q Zhang, Q Zhu, W Liu, Y Chen, R Qiu, B Wang… - Molecular plant, 2015 - cell.com
CRISPR/Cas9 genome targeting systems have been applied to a variety of species. However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …
Q Liu, C Zhao, K Sun, Y Deng, Z Li - Molecular Plant, 2023 - cell.com
CRISPR/Cas genome-editing tools provide unprecedented opportunities for basic plant biology research and crop breeding. However, the lack of robust delivery methods has …
Z Ali, A Abulfaraj, A Idris, S Ali, M Tashkandi… - Genome biology, 2015 - Springer
Abstract Background The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently …
X Ma, Q Zhu, Y Chen, YG Liu - Molecular plant, 2016 - cell.com
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …
Increasing agricultural productivity is critical to feed the ever-growing human population. Being linked intimately to plant health, growth and productivity, harnessing the plant …
Plant pathogens pose a major threat to crop productivity. Typically, phytopathogens exploit plants' susceptibility (S) genes to facilitate their proliferation. Disrupting these S genes may …