During assembly, ribosomal particles in bacteria fold according to energy landscapes
comprised of multiple parallel pathways. Cryo-electron microscopy studies have identified a …

Structural DNA nanotechnology is beginning to emerge as a widely accessible research tool
to mechanistically study diverse biophysical processes. Enabled by scaffolded DNA origami …

Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that
involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) …

Ribosome biogenesis requires auxiliary factors to promote folding and assembly of
ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) …

Ribosomes accurately decode mRNA by proofreading each aminoacyl-tRNA that is
delivered by the elongation factor EF-Tu. To understand the molecular mechanism of this …

In contrast to the bacterial translation machinery, mitoribosomes and mitochondrial
translation factors are highly divergent in terms of composition and architecture. There is …

Understanding the assembly principles of biological macromolecular complexes remains a
significant challenge, due to the complexity of the systems and the difficulties in developing …

Assembly of the mitoribosome is largely enigmatic and involves numerous assembly factors.
Little is known about their function and the architectural transitions of the pre-ribosomal …

Ribosome biogenesis is an elaborate and energetically expensive program that involve two
hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one …

Mitoribosomes consist of ribosomal RNA and protein components, coordinated assembly of
which is critical for function. We used mitoribosomes from Trypanosoma brucei with reduced …