Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

Enzyme catalysis is gaining increasing importance in synthetic chemistry. Nowadays, the
growing number of biocatalysts accessible by means of bioinformatics and enzyme …

Until recently, glycosidases, naturally hydrolyzing carbohydrate-active enzymes, have found
few synthetic applications in industry, being primarily used for cleaving unwanted …

Bottom-up synthesis of β-glucans such as callose, fungal β-(1, 3)(1, 6)-glucan and cellulose,
can create the defined compounds that are needed to perform fundamental studies on …

Azido sugars have found frequent use as probes of biological systems in approaches
ranging from cell surface metabolic labeling to activity-based proteomic profiling of …

Highlights•Carbohydrate-active enzymes (CAZymes) are invaluable tools for the synthesis of
glycoconjugates.•Manipulation of the glycosidic linkage (synthesis and hydrolysis) are …

An increasing number of transglycosylase-based processes provide access to
oligosaccharides or glycoconjugates, some of them reaching performance levels compatible …

Glycosides are widely used in many fields due to their favorable biological activity. The
traditional plant extractions and chemical methods for glycosides production are limited by …

Highlights•Glycoengineering of glycans and glycoconjugates requires access to better
enzymes.•Carbohydrate-Active enZymes can be improved using novel protein engineering …

Carbohydrate-active enzymes (CAZymes) are responsible for the biosynthesis, modification
and degradation of all glycans in Nature. Advances in genomic and metagenomic …