Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
[HTML][HTML] Recent advances and perspectives of nucleic acid detection for coronavirus
M Shen, Y Zhou, J Ye, AAA Al-Maskri, Y Kang… - Journal of …, 2020 - Elsevier
The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a
great threat to global public health. Therefore, rapid and accurate identification of …
great threat to global public health. Therefore, rapid and accurate identification of …
Principles and applications of nucleic acid strand displacement reactions
Dynamic DNA nanotechnology, a subfield of DNA nanotechnology, is concerned with the
study and application of nucleic acid strand-displacement reactions. Strand-displacement …
study and application of nucleic acid strand-displacement reactions. Strand-displacement …
Loop-mediated isothermal amplification (LAMP)–review and classification of methods for sequence-specific detection
L Becherer, N Borst, M Bakheit, S Frischmann… - Analytical …, 2020 - pubs.rsc.org
In the course of the last 20 years, isothermal nucleic acid amplification tests have emerged
as an important diagnostic tool, not only for clinical applications, but also for food quality …
as an important diagnostic tool, not only for clinical applications, but also for food quality …
[HTML][HTML] Research advances in the detection of miRNA
J Ye, M Xu, X Tian, S Cai, S Zeng - Journal of pharmaceutical analysis, 2019 - Elsevier
MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in
length), noncoding, functional RNAs. With the development of molecular biology, the …
length), noncoding, functional RNAs. With the development of molecular biology, the …
Point-of-use rapid detection of SARS-CoV-2: nanotechnology-enabled solutions for the COVID-19 pandemic
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the COVID-19
pandemic that has been spreading around the world since December 2019. More than 10 …
pandemic that has been spreading around the world since December 2019. More than 10 …
Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification
JC Rolando, E Jue, JT Barlow… - Nucleic acids …, 2020 - academic.oup.com
Isothermal amplification assays, such as loop-mediated isothermal amplification (LAMP),
show great utility for the development of rapid diagnostics for infectious diseases because …
show great utility for the development of rapid diagnostics for infectious diseases because …
Reduced false positives and improved reporting of loop-mediated isothermal amplification using quenched fluorescent primers
P Hardinge, JAH Murray - Scientific reports, 2019 - nature.com
Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular
diagnostics as an alternative to PCR based methods. There are numerous reported …
diagnostics as an alternative to PCR based methods. There are numerous reported …
Real-time detection and monitoring of loop mediated amplification (LAMP) reaction using self-quenching and de-quenching fluorogenic probes
VJ Gadkar, DM Goldfarb, S Gantt, PAG Tilley - Scientific reports, 2018 - nature.com
Loop-mediated isothermal amplification (LAMP) is an isothermal nucleic acid amplification
(iNAAT) technique known for its simplicity, sensitivity and speed. Its low-cost feature has …
(iNAAT) technique known for its simplicity, sensitivity and speed. Its low-cost feature has …
Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA
RJ Meagher, A Priye, YK Light, C Huang, E Wang - Analyst, 2018 - pubs.rsc.org
Loop-mediated isothermal amplification (LAMP), coupled with reverse transcription (RT),
has become a popular technique for detection of viral RNA due to several desirable …
has become a popular technique for detection of viral RNA due to several desirable …