Challenges and Opportunities for Immunoprofiling Using a Spatial High-Plex Technology: The NanoString GeoMx® Digital Spatial Profiler

S Hernandez, R Lazcano, A Serrano, S Powell… - Frontiers in …, 2022 - frontiersin.org
Characterization of the tumor microenvironment through immunoprofiling has become an
essential resource for the understanding of the complex immune cell interactions and the …

Developing image analysis methods for digital pathology

P Bankhead - The Journal of pathology, 2022 - Wiley Online Library
The potential to use quantitative image analysis and artificial intelligence is one of the
driving forces behind digital pathology. However, despite novel image analysis methods for …

Companion diagnostic requirements for spatial biology using multiplex immunofluorescence and multispectral imaging

D Locke, CC Hoyt - Frontiers in Molecular Biosciences, 2023 - frontiersin.org
Immunohistochemistry has long been held as the gold standard for understanding the
expression patterns of therapeutically relevant proteins to identify prognostic and predictive …

LOXL4 Shuttled by Tumor Cells–derived Extracellular Vesicles Promotes Immune Escape in Hepatocellular Carcinoma by Activating the STAT1/PD-L1 Axis

L Zhao, R Pei, Y Ding, Z Su, D Li, S Zhu… - Journal of …, 2024 - journals.lww.com
Emerging evidence has validated that extracellular vesicles (EVs) regulate hepatocellular
carcinoma (HCC) progression, while its role in HCC immune escape remains to be …

[HTML][HTML] Addressing persistent challenges in digital image analysis of cancerous tissues

S Prabhakaran, C Yapp, GJ Baker, J Beyer, YH Chang… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Abstract The National Cancer Institute (NCI) supports many research programs and
consortia, many of which use imaging as a major modality for characterizing cancerous …

Quantitative image analysis pipeline for detecting circulating hybrid cells in immunofluorescence images with human‐level accuracy

RT Heussner, RM Whalen, A Anderson… - Cytometry Part …, 2024 - Wiley Online Library
Circulating hybrid cells (CHCs) are a newly discovered, tumor‐derived cell population found
in the peripheral blood of cancer patients and are thought to contribute to tumor metastasis …

Quantitative image analysis pipeline for detecting circulating hybrid cells in immunofluorescence images with human-level accuracy

RT Heussner, RM Whalen, A Anderson, H Theison… - bioRxiv, 2023 - biorxiv.org
Circulating hybrid cells (CHCs) are a newly discovered, tumor-derived cell population
identified in the peripheral blood of cancer patients and are thought to contribute to tumor …

Spatial Technologies: A Game Changer for Studying the Tumor Microenvironment

RY LEE, MC LAU, D GOH, F WEE, JCT LIM… - Handbook of Cancer …, 2023 - Springer
Moving away from the generic treatment for all cancer, precision medicine provides a
targeted therapy based on the different profile presented. Since the signing of Precision …