Recent developments in isolating methods for exosomes

J Gao, A Li, J Hu, L Feng, L Liu, Z Shen - Frontiers in bioengineering …, 2023 - frontiersin.org
Exosomes are the smallest extracellular vesicles that can be released by practically all cell
types, and range in size from 30 nm to 150 nm. As the major marker of liquid biopsies …

Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

H Zhang, L Cao, J Brodsky, I Gablech, F Xu, Z Li… - TrAC Trends in …, 2024 - Elsevier
This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …

A droplet-based multivolume microfluidic device for digital polymerase chain reaction

C Wei, C Yu, S Li, J Meng, T Li, J Cheng, J Li - Sensors and Actuators B …, 2022 - Elsevier
Digital PCR plays an essential role in detecting and quantifying nucleic acid targets. At the
same time, there are still challenges in its applications, including reduction of cost …

Advances in improvement strategies of digital nucleic acid amplification for pathogen detection

X Xiang, Y Shang, J Zhang, Y Ding, Q Wu - TrAC Trends in Analytical …, 2022 - Elsevier
Digital nucleic acid amplification technologies (dNAATs) have become an emerging platform
for pathogen detection. It is not only suitable for the absolute quantitative analysis of target …

Multiplex digital PCR with digital melting curve analysis on a self-partitioning SlipChip

Y Yu, Z Yu, X Pan, L Xu, R Guo, X Qian, F Shen - Analyst, 2022 - pubs.rsc.org
Digital polymerase chain reaction (digital PCR) can provide absolute quantification of target
nucleic acids with high sensitivity, excellent precision, and superior resolution. Digital PCR …

Recent advances in droplet‐based microfluidics in liquid biopsy for cancer diagnosis

J Shi, Y Zhang, Y Fan, Y Liu, M Yang - Droplet, 2024 - Wiley Online Library
Liquid biopsy, a noninvasive technique to obtain tumor information from body fluids, is an
emerging technology for cancer diagnosis, prognosis, and monitoring, providing crucial …

A low-cost, programmable, and multi-functional droplet printing system for low copy number SARS-CoV-2 digital PCR determination

W Bu, W Li, J Li, T Ao, Z Li, B Wu, S Wu, W Kong… - Sensors and Actuators B …, 2021 - Elsevier
Droplet digital polymerase chain reaction (ddPCR) is a rapidly developing technology used
for accurate, quantitative analysis of rare samples. However, ddPCR has only been …

Precise monodisperse droplet generation by pressure-driven microfluidic flows

W Zeng, S Yang, Y Liu, T Yang, Z Tong, X Shan… - Chemical engineering …, 2022 - Elsevier
In a T-junction microdroplet generator, as monodisperse droplets are formed for the droplet
digital polymerase chain reaction (PCR), a mathematical model which can describe the …

Deep‐dLAMP: Deep Learning‐Enabled Polydisperse Emulsion‐Based Digital Loop‐Mediated Isothermal Amplification

L Chen, J Ding, H Yuan, C Chen, Z Li - Advanced Science, 2022 - Wiley Online Library
Digital nucleic acid amplification tests enable absolute quantification of nucleic acids, but the
generation of uniform compartments and reading of the fluorescence requires specialized …

Smartphone integrated handheld (SPEED) digital polymerase chain reaction device

X Liu, X Wang, H Zhang, Z Yan, M Gaňová… - Biosensors and …, 2023 - Elsevier
We demonstrate a smartphone integrated handheld (SPEED) digital polymerase chain
reaction (dPCR) device for point-of-care application. The device has dimensions of≈ 100× …