YIP vectors Transplacement Integration into reiterated DNA Transposition vectors
Transcriptional promoters and terminators Foreign versus yeast promoters Glycolytic …

The field of gene therapy has evolved over the past two decades after the first introduction of
nucleic acid drugs, such as plasmid DNA (pDNA). With the development of in vitro …

In order to generate recombinant bovine respiratory syncytial virus (BRSV), the genome of
BRSV strain A51908, variant ATue51908, was cloned as cDNA. We provide here the …

Expression of the cystic fibrosis transmembrane conductance regulator (CFTR) generates
adenosine 3′, 5′-monophosphate (cAMP)-regulated chloride channels, indicating that …

The internal ribosome entry site (IRES) has been widely used to coexpress heterologous
gene products by a message from a single promoter. However, little is known about the …

Glycogen synthase kinase-3 (GSK-3), a protein-serine kinase implicated in cell-fate
determination and differentiation, phosphorylates several regulatory proteins that are …

CFTR, the protein associated with cystic fibrosis, is phosphorylated on serine residues in
response to CAMP agonists. Serines 660, 737, 795, and 613 were identified as in vivo …

Hepatitis C virus (HCV) is the major cause of transfusion-acquired non-A, non-B hepatitis.
HCV is an enveloped positive-sense RNA virus which has been classified as a new genus …

[57] ABSTRACT A reagent for introducing a nucleic acid into an animal cell. The reagent
comprises (a) a neutral lipid such as dioleoylphosphatidylethanolamine and (b) a cationic …

THE cystic fibrosis transmembrane conductance regulator (CFTR) is a phosphorylation-
regulated Cl-channel located in the apical membrane of epithelia1–10. Although cystic …