The algal chloroplast as a testbed for synthetic biology designs aimed at radically rewiring plant metabolism

HO Jackson, HN Taunt, PM Mordaka… - Frontiers in Plant …, 2021 - frontiersin.org
Sustainable and economically viable support for an ever-increasing global population
requires a paradigm shift in agricultural productivity, including the application of …

Chloroplast ATP synthase: From structure to engineering

T Rühle, D Leister, V Pasch - The Plant Cell, 2024 - academic.oup.com
F-type ATP synthases are extensively researched protein complexes because of their
widespread and central role in energy metabolism. Progress in structural biology …

Chloroplast ATP synthase biogenesis requires peripheral stalk subunits AtpF and ATPG and stabilization of atpE mRNA by OPR protein MDE1

F Chaux, D Jarrige, M Rodrigues‐Azevedo… - The Plant …, 2023 - Wiley Online Library
Chloroplast ATP synthase contains subunits of plastid and nuclear genetic origin. To
investigate the coordinated biogenesis of this complex, we isolated novel ATP synthase …

Environmental and nuclear influences on microalgal chloroplast gene expression

SE Carrera-Pacheco, B Hankamer, M Oey - Trends in Plant Science, 2023 - cell.com
Microalgal chloroplasts, such as those of the model organism Chlamydomonas reinhardtii,
are emerging as a new platform to produce recombinant proteins, including industrial …

Types and functions of mitoribosome-specific ribosomal proteins across eukaryotes

V Scaltsoyiannes, N Corre, F Waltz… - International Journal of …, 2022 - mdpi.com
Mitochondria are key organelles that combine features inherited from their bacterial
endosymbiotic ancestor with traits that arose during eukaryote evolution. These energy …

Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast

K Ma, L Deng, H Wu, J Fan - Bioresources and Bioprocessing, 2022 - Springer
Microalgae are cosmopolitan organisms in nature with short life cycles, playing a
tremendous role in reducing the pressure of industrial carbon emissions. Besides …

Pas de Trois: An Overview of Penta-, Tetra-, and Octo-Tricopeptide Repeat Proteins From Chlamydomonas reinhardtii and Their Role in Chloroplast Gene Expression

KS Macedo-Osorio, A Martínez-Antonio… - Frontiers in Plant …, 2021 - frontiersin.org
Penta-, Tetra-, and Octo-tricopeptide repeat (PPR, TPR, and OPR) proteins are nucleus-
encoded proteins composed of tandem repeats of 35, 34, and 38–40 amino acids …

Light and heat-shock mediated TDA1 overexpression as a tool for controlled high-yield recombinant protein production in Chlamydomonas reinhardtii chloroplasts

SE Carrera-Pacheco, B Hankamer, M Oey - Algal research, 2020 - Elsevier
The microalga Chlamydomonas reinhardtii offers a rapid, scalable and low-cost platform for
recombinant protein production. Its chloroplast provides a particularly robust expression …

The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo in Chlamydomonas reinhardtii

SI Ozawa, M Cavaiuolo, D Jarrige, R Kuras… - The Plant …, 2020 - academic.oup.com
In the green alga Chlamydomonas (Chlamydomonas r einhardtii), chloroplast gene
expression is tightly regulated posttranscriptionally by gene-specific trans-acting protein …

Use of plant chloroplast RNA-binding proteins as orthogonal activators of chloroplast transgenes in the green alga Chlamydomonas reinhardtii

N Shahar, T Elman, R Williams-Carrier, O Ben-Zvi… - Algal Research, 2021 - Elsevier
The genomes of microalgal chloroplasts offer many advantages as a scaffold for the
expression of foreign proteins for applications in biotechnology. To realize this potential, it …