[HTML][HTML] An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology
Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
Structure and transport mechanism of the sodium/proton antiporter MjNhaP1
C Paulino, D Wöhlert, E Kapotova, Ö Yildiz… - Elife, 2014 - elifesciences.org
Sodium/proton antiporters are essential for sodium and pH homeostasis and play a major
role in human health and disease. We determined the structures of the archaeal …
role in human health and disease. We determined the structures of the archaeal …
Proteoliposomes–a system to study membrane proteins under buffer gradients by cryo-EM
Membrane proteins are vital to life and major therapeutic targets. Yet, understanding how
they function is limited by a lack of structural information. In biological cells, membrane …
they function is limited by a lack of structural information. In biological cells, membrane …
Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1
Cyclic nucleotide-modulated ion channels are important for signal transduction and
pacemaking in eukaryotes. The molecular determinants of ligand gating in these channels …
pacemaking in eukaryotes. The molecular determinants of ligand gating in these channels …
Cryo-electron microscopy of membrane proteins
KN Goldie, P Abeyrathne, F Kebbel, M Chami… - … : Methods and Protocols, 2014 - Springer
Electron crystallography is used to study membrane proteins in the form of planar, two-
dimensional (2D) crystals, or other crystalline arrays such as tubular crystals. This method …
dimensional (2D) crystals, or other crystalline arrays such as tubular crystals. This method …
pH-and sodium-induced changes in a sodium/proton antiporter
C Paulino, W Kühlbrandt - Elife, 2014 - elifesciences.org
We examined substrate-induced conformational changes in MjNhaP1, an archaeal
electroneutral Na+/H+-antiporter resembling the human antiporter NHE1, by electron …
electroneutral Na+/H+-antiporter resembling the human antiporter NHE1, by electron …
High-resolution cryoelectron microscopy structure of the cyclic nucleotide-modulated potassium channel MloK1 in a lipid bilayer
Eukaryotic cyclic nucleotide-modulated channels perform their diverse physiological roles
by opening and closing their pores to ions in response to cyclic nucleotide binding. We here …
by opening and closing their pores to ions in response to cyclic nucleotide binding. We here …
[HTML][HTML] 2dx_automator: Implementation of a semiautomatic high-throughput high-resolution cryo-electron crystallography pipeline
The introduction of direct electron detectors (DED) to cryo-electron microscopy has
tremendously increased the signal-to-noise ratio (SNR) and quality of the recorded images …
tremendously increased the signal-to-noise ratio (SNR) and quality of the recorded images …
[HTML][HTML] Single particle 3D reconstruction for 2D crystal images of membrane proteins
In cases where ultra-flat cryo-preparations of well-ordered two-dimensional (2D) crystals are
available, electron crystallography is a powerful method for the determination of the high …
available, electron crystallography is a powerful method for the determination of the high …
[HTML][HTML] Image processing techniques for high-resolution structure determination from badly ordered 2D crystals
Abstract 2D electron crystallography can be used to study small membrane proteins in their
native environment. Obtaining highly ordered 2D crystals is difficult and time-consuming …
native environment. Obtaining highly ordered 2D crystals is difficult and time-consuming …