Isolation of plant nuclei for estimation of nuclear DNA content: overview and best practices
A critical aspect for obtaining accurate, reliable, and high‐resolution estimates of nuclear
DNA content is the release of nuclei from the cytoplasm in sufficient amounts, while …
DNA content is the release of nuclei from the cytoplasm in sufficient amounts, while …
Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status
L D'hondt, M Höfte, E Van Bockstaele… - Molecular Plant …, 2011 - Wiley Online Library
Flow cytometers are probably the most multipurpose laboratory devices available. They can
analyse a vast and very diverse range of cell parameters. This technique has left its mark on …
analyse a vast and very diverse range of cell parameters. This technique has left its mark on …
Induction and Flow Cytometry Identification of Tetraploids from Seed‐Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don
The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine
induction from seeds explants, and the ploidy of the plants was identified by flow cytometry …
induction from seeds explants, and the ploidy of the plants was identified by flow cytometry …
DNA content variation in monilophytes and lycophytes: large genomes that are not endopolyploid
JD Bainard, TA Henry, LD Bainard… - Chromosome Research, 2011 - Springer
Less than 1% of known monilophytes and lycophytes have a genome size estimate, and
substantially less is known about the presence and prevalence of endopolyploid nuclei in …
substantially less is known about the presence and prevalence of endopolyploid nuclei in …
Genome size and endopolyploidy evolution across the moss phylogeny
Abstract Background and Aims Compared with other plant lineages, bryophytes have very
small genomes with little variation across species, and high levels of endopolyploid nuclei …
small genomes with little variation across species, and high levels of endopolyploid nuclei …
Deep ultraviolet mapping of intracellular protein and nucleic acid in femtograms per pixel
MC Cheung, JG Evans, B McKenna… - Cytometry Part …, 2011 - Wiley Online Library
By using imaging spectrophotometry with paired images in the 200‐to 280‐nm wavelength
range, we have directly mapped intracellular nucleic acid and protein distributions across a …
range, we have directly mapped intracellular nucleic acid and protein distributions across a …
The effects of rapid desiccation on estimates of plant genome size
Flow cytometry has become the dominant method for estimating nuclear DNA content in
plants, either for ploidy determination or quantification of absolute genome size. Current best …
plants, either for ploidy determination or quantification of absolute genome size. Current best …
The genome size of clusterbean (Cyamopsis tetragonoloba) is significantly smaller compared to its wild relatives as estimated by flow cytometry
Clusterbean (C. tetragonoloba) is an important, leguminous vegetable and industrial crop
with vast genetic diversity but meager genetic, cytological and genomic information. In the …
with vast genetic diversity but meager genetic, cytological and genomic information. In the …
Flow cytometry in plant research: a success story
J Vrána, P Cápal, M Bednářová, J Doležel - Applied Plant Cell Biology …, 2014 - Springer
Flow cytometry is a powerful technique with numerous applications in biomedical research,
including immunology, haematology, oncology and other fields. It has also found important …
including immunology, haematology, oncology and other fields. It has also found important …
Nuclear DNA content of Pongamia pinnata L. and genome size stability of in vitro-regenerated plantlets
RR Choudhury, S Basak, AM Ramesh, L Rangan - Protoplasma, 2014 - Springer
Pongamia pinnata L. is a multipurpose versatile legume that is well known as a prospective
feedstock biodiesel species. However, to date, there has been little genomic research aimed …
feedstock biodiesel species. However, to date, there has been little genomic research aimed …