Mesenchymal stem cell-derived exosomal microRNA-182-5p alleviates myocardial ischemia/reperfusion injury by targeting GSDMD in mice

R Yue, S Lu, Y Luo, J Zeng, H Liang, D Qin… - Cell death …, 2022 - nature.com
Recent evidence indicates that exosomes derived from mesenchymal stem cells (MSCs)
confer protective effects against myocardial ischemia/reperfusion (I/R) injury. Exosomes are …

[HTML][HTML] TATA box binding protein and ribosomal protein 4 are suitable reference genes for normalization during quantitative polymerase chain reaction study in …

SJ Jang, RH Jeon, HD Kim, JC Hwang… - … journal of animal …, 2020 - ncbi.nlm.nih.gov
Objective Quantitative polymerase chain reaction (qPCR) has been extensively used in the
field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical …

PPIA, HPRT1, and YWHAZ genes are suitable for normalization of mRNA expression in long‐term expanded human mesenchymal stem cells

RH Jeon, WJ Lee, YB Son, D Bharti… - BioMed Research …, 2019 - Wiley Online Library
Long‐term expansion of mesenchymal stem cells (MSCs) under defined culture conditions
is necessary in human stem cell therapy. However, it alters the characteristics of MSCs …

Human umbilical cord-mesenchymal stem cells-derived exosomes carrying microRNA-15a-5p possess therapeutic effects on Wilms tumor via regulating septin 2

H Huang, P Zhong, J Zhang, X Chen, J Chen, T Lin… - …, 2022 - Taylor & Francis
The exact mechanism of miR-15a-5p shuttled by human umbilical cord-mesenchymal stem
cell-derived exosomes (hUC-MSCs-Exo) in Wilms tumor (WT) was estimated. WT tissues …

Optimizations for identifying reference genes in bone and cartilage bioengineering

F Xiong, X Cheng, C Zhang, RM Klar, T He - BMC biotechnology, 2021 - Springer
Background Reverse transcription quantitative real-time polymerase chain reaction (RT-
qPCR) remains one of the best-established techniques to assess gene expression patterns …

HPRT1 Most Suitable Reference Gene for Accurate Normalization of mRNA Expression in Canine Dermal Tissues with Radiation Therapy

SY Lee, YH Choe, JH Han, G Hwang, MY Choi… - Genes, 2022 - mdpi.com
Reference genes are crucial in molecular biological studies as an internal control for gene
re-search as they exhibit consistent expression patterns across many tissue types. In …

Selection of reference genes for quantitative real-time polymerase chain reaction in porcine embryos

WJ Lee, SJ Jang, SC Lee, JS Park… - Reproduction …, 2017 - CSIRO Publishing
To study gene expression and to determine distinctive characteristics of embryos produced
by different methods, normalisation of the gene (s) of interest against reference gene (s) has …

Evaluation of the stability of standard reference genes of adipose-derived mesenchymal stem cells during in vitro proliferation and differentiation

FB Ayanoğlu, AE Elçin, YM Elçin - Molecular Biology Reports, 2020 - Springer
Mesenchymal stem cells (MSCs) from a variety of sources are being used in pre-clinical and
clinical studies. The choice of optimal source for treatment of diseases requires quantitative …

IFN‐γ Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation‐Induced Porcine Bone Marrow‐Derived Mesenchymal …

HJ Lee, HD Kim, CH Jo, EY Bok, SB Kim… - BioMed Research …, 2021 - Wiley Online Library
IFN‐γ licensing to mesenchymal stem cells (MSCs) is applied to enhance the therapeutic
potential of MSCs. However, although the features of MSCs are affected by several stimuli …

[HTML][HTML] PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic–pituitary–gonadal axis in sows

HD Kim, CH Jo, YH Choe, HJ Lee, M Jang… - Animal …, 2022 - ncbi.nlm.nih.gov
Objective The quantitative reverse transcription polymerase chain reaction (qPCR) is the
most accurate and reliable technique for analysis of gene expression. Endogenous …