Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Droplet microfluidics generates and manipulates discrete droplets through immiscible
multiphase flows inside microchannels. Due to its remarkable advantages, droplet …

Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

The advent of soft lithography allowed for an unprecedented expansion in the field of
microfluidics. However, the vast majority of PDMS microfluidic devices are still made with …

The genetic, functional or compositional heterogeneity of healthy and diseased tissues
presents major challenges in drug discovery and development. Such heterogeneity hinders …

We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

Bacteria primarily exist in robust, surface-associated communities known as biofilms,
ubiquitous in both natural and anthropogenic environments. Mature biofilms resist a wide …

The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Surfactants are an essential part of the droplet-based microfluidic technology. They are
involved in the stabilization of droplet interfaces, in the biocompatibility of the system and in …

Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …