Functional single-cell analyses: flow cytometry and cell sorting of microbial populations and communities

S Müller, G Nebe-von-Caron - FEMS Microbiology reviews, 2010 - academic.oup.com
The still poorly explored world of microbial functioning is about to be uncovered by a
combined application of old and new technologies. Bacteria, especially, are still in the dark …

Past, present and future applications of flow cytometry in aquatic microbiology

Y Wang, F Hammes, K De Roy, W Verstraete… - Trends in …, 2010 - cell.com
Flow cytometry (FCM) is rapidly becoming an essential tool in the field of aquatic
microbiology. It provides opportunities for microbial analysis at both the community and …

[图书][B] Microwave-assisted extraction for bioactive compounds: theory and practice

F Chemat, G Cravotto - 2012 - books.google.com
With increasing energy prices and the drive to reduce CO2 emissions, food industries are
challenged to find new technologies in order to reduce energy consumption, to meet legal …

Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities

JM Gasol, PA Del Giorgio - Scientia Marina, 2000 - scientiamarina.revistas.csic.es
Flow cytometry is rapidly becoming a routine methodology in aquatic microbial ecology. The
combination of simple to use bench-top flow cytometers and highly fluorescent nucleic acid …

Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems?

P Lebaron, P Servais, H Agogué… - Applied and …, 2001 - Am Soc Microbiol
The nucleic acid contents of individual bacterial cells as determined with three different
nucleic acid-specific fluorescent dyes (SYBR I, SYBR II, and SYTO 13) and flow cytometry …

Current and future applications of flow cytometry in aquatic microbiology

J Vives-Rego, P Lebaron… - FEMS microbiology …, 2000 - academic.oup.com
Flow cytometry has become a valuable tool in aquatic and environmental microbiology that
combines direct and rapid assays to determine numbers, cell size distribution and additional …

A comparative study of the cytometric characteristics of high and low nucleic‐acid bacterioplankton cells from different aquatic ecosystems

T Bouvier, PA Del Giorgio… - Environmental …, 2007 - Wiley Online Library
Flow cytometry has revealed the existence of two distinct fractions of bacterioplankton cells,
characterized by high and low nucleic acid contents (HNA and LNA cells). Although these …

Flow cytometric assessment of bacterial abundance in soils, sediments and sludge

A Frossard, F Hammes, MO Gessner - Frontiers in microbiology, 2016 - frontiersin.org
Bacterial abundance is a fundamental measure in microbiology, but its assessment is often
tedious, especially for soil, and sediment samples. To overcome this limitation, we adopted a …

Variations of bacterial-specific activity with cell size and nucleic acid content assessed by flow cytometry

P Lebaron, P Servais, AC Baudoux, M Bourrain… - Aquatic Microbial …, 2002 - int-res.com
In most aquatic bacterial communities, it is possible to discriminate bacterial cells with a high
nucleic acid content (HNA) from those with a low nucleic acid content (LNA) by flow …

Physiological structure and single‐cell activity in marine bacterioplankton

PA Del Giorgio, JM Gasol - Microbial ecology of the oceans, 2008 - Wiley Online Library
Generations of microbiologists have pondered over the issue of bacterial activity and
whether most bacteria in the environment are “active,”“inactive,” or “dormant”(Stevenson …