Gene editing stands for the methods to precisely make changes to a specific nucleic acid
sequence. With the recent development of the clustered regularly interspaced short …

Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …

Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …

CRISPOR. org is a web tool for genome editing experiments with the CRISPR–Cas9 system.
It finds guide RNAs in an input sequence and ranks them according to different scores that …

The arrangement (syntax) of transcription factor (TF) binding motifs is an important part of the
cis-regulatory code, yet remains elusive. We introduce a deep learning model, BPNet, that …

CRISPR-Cas9 genome editing has potential to cure diseases without current treatments, but
therapies must be safe. Here we show that CRISPR-Cas9 editing can introduce unintended …

INTRODUCTION To combat invading pathogens, cells develop an adaptive immune
response by changing their own genetic information. In vertebrates, the generation of …

Background The success of the CRISPR/Cas9 genome editing technique depends on the
choice of the guide RNA sequence, which is facilitated by various websites. Despite the …

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-
AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in …

A major challenge for effective application of CRISPR systems is to accurately predict the
single guide RNA (sgRNA) on-target knockout efficacy and off-target profile, which would …