Interfacing coacervates with membranes: from artificial organelles and hybrid protocells to intracellular delivery

T Lu, S Javed, C Bonfio, E Spruijt - Small methods, 2023 - Wiley Online Library
Compartmentalization is crucial for the functioning of cells. Membranes enclose and protect
the cell, regulate the transport of molecules entering and exiting the cell, and organize …

Synthetic intracellular environments: from basic science to applications

W Zong, X Shao, J Li, Y Chai, X Hu… - Analytical chemistry, 2023 - ACS Publications
The intracellular environment is a common feature of all living cells. It plays an active role in
promoting and regulating intracellular reactions. The construction of an artificial intracellular …

Shocker─ A Molecular Dynamics Protocol and Tool for Accelerating and Analyzing the Effects of Osmotic Shocks

MPA van Tilburg, SJ Marrink, M König… - Journal of Chemical …, 2023 - ACS Publications
The process of osmosis, a fundamental phenomenon in life, drives water through a
semipermeable membrane in response to a solute concentration gradient across this …

Physical Concept to Explain the Regulation of Lipid Membrane Phase Separation under Isothermal Conditions

N Shimokawa, T Hamada - Life, 2023 - mdpi.com
Lateral phase separation within lipid bilayer membranes has attracted considerable
attention in the fields of biophysics and cell biology. Living cells organize laterally …

Light-switchable membrane permeability in giant unilamellar vesicles

P Albanese, S Cataldini, CZJ Ren, N Valletti, J Brunetti… - Pharmaceutics, 2022 - mdpi.com
In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural
phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a …

Osmotic spawning vesicle

M Kurisu, M Imai - Soft Matter, 2024 - pubs.rsc.org
We discovered a cascade vesicle division system driven by osmotic inflation. Binary giant
unilamellar vesicles (GUVs) composed of sodium bis (2-ethylhexyl) sulfosuccinate (AOT) …

Temporal pH waveforms generated in an enzymatic reaction network in batch and cell-sized microcompartments

M Itatani, G Holló, P Albanese, N Valletti… - Cell Reports Physical …, 2025 - cell.com
Investigating and designing reaction networks and their emergent phenomena are the
backbone of systems chemistry. Designing chemical oscillators in homogeneous systems …

Significance of in situ quantitative membrane property–morphology relation (QmPMR) analysis

Z Nicolella, Y Okamoto, NM Watanabe, GL Thompson… - Soft Matter, 2024 - pubs.rsc.org
Deformation of the cell membrane is well understood from the viewpoint of protein
interactions and free energy balance. However, the various dynamic properties of the …

Energy Transfer on Cytoskeleton of Red Blood Cell Ghosts and their Efficiency Control by KCl Concentration‐induced Cell Shrinkage

H Hiroe, M Kawamoto, H Imamura… - … –A European Journal, 2024 - Wiley Online Library
Lipid bilayer membranes such as liposomes have been utilized as platforms for bioinspired
artificial photosynthesis. Embedding functional compounds, including chromophores and …

Models of Protocells Undergoing Asymmetrical Division

M Villani, E Alboresi, R Serra - Entropy, 2024 - mdpi.com
The conditions that allow for the sustained growth of a protocell population are investigated
in the case of asymmetrical division. The results are compared to those of previous studies …