Soil microbiome engineering for sustainability in a changing environment

JK Jansson, R McClure, RG Egbert - Nature Biotechnology, 2023 - nature.com
Recent advances in microbial ecology and synthetic biology have the potential to mitigate
damage caused by anthropogenic activities that are deleteriously impacting Earth's soil …

Cell-free gene expression: an expanded repertoire of applications

AD Silverman, AS Karim, MC Jewett - Nature Reviews Genetics, 2020 - nature.com
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

Stepping on the gas to a circular economy: accelerating development of carbon-negative chemical production from gas fermentation

N Fackler, BD Heijstra, BJ Rasor… - Annual review of …, 2021 - annualreviews.org
Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change
poses significant environmental, economic, and social challenges globally. Technologies …

High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration

JR Elmore, GN Dexter, H Baldino, JD Huenemann… - Science …, 2023 - science.org
Efficient genome engineering is critical to understand and use microbial functions. Despite
recent development of tools such as CRISPR-Cas gene editing, efficient integration of …

Bottom-up construction of complex biomolecular systems with cell-free synthetic biology

N Laohakunakorn, L Grasemann… - … in bioengineering and …, 2020 - frontiersin.org
Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …

Translating new synthetic biology advances for biosensing into the earth and environmental sciences

I Del Valle, EM Fulk, P Kalvapalle, JJ Silberg… - Frontiers in …, 2021 - frontiersin.org
The rapid diversification of synthetic biology tools holds promise in making some classically
hard-to-solve environmental problems tractable. Here we review longstanding problems in …

Programmable CRISPR‐Cas transcriptional activation in bacteria

HI Ho, JR Fang, J Cheung, HH Wang - Molecular systems biology, 2020 - embopress.org
Programmable gene activation enables fine‐tuned regulation of endogenous and synthetic
gene circuits to control cellular behavior. While CRISPR‐Cas‐mediated gene activation has …

Cell-free gene expression: methods and applications

AC Hunt, BJ Rasor, K Seki, HM Ekas, KF Warfel… - Chemical …, 2024 - ACS Publications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Genetic and metabolic engineering challenges of C1-gas fermenting acetogenic chassis organisms

B Bourgade, NP Minton, MA Islam - FEMS Microbiology …, 2021 - academic.oup.com
Unabated mining and utilisation of petroleum and petroleum resources and their conversion
to essential fuels and chemicals have drastic environmental consequences, contributing to …

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

SD Cole, AE Miklos, AC Chiao, ZZ Sun… - Synthetic and Systems …, 2020 - Elsevier
Cell-free systems that mimic essential cell functions, such as gene expression, have
dramatically expanded in recent years, both in terms of applications and widespread …