Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements
J Kaur, A Kumar, J Kaur - International journal of biological …, 2018 - Elsevier
E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …
and the availability of improved genetic tools/methods are making it more valuable than …
Strategies for improving antimicrobial peptide production
Antimicrobial peptides (AMPs) found in a wide range of animal, insect, and plant species are
host defense peptides forming an integral part of their innate immunity. Although the exact …
host defense peptides forming an integral part of their innate immunity. Although the exact …
Fusion tags to enhance heterologous protein expression
MR Ki, SP Pack - Applied Microbiology and Biotechnology, 2020 - Springer
Escherichia coli is the most widely used heterologous protein expression system. However,
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …
Current strategies for protein production and purification enabling membrane protein structural biology
Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …
Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry
MM Gomari, N Saraygord-Afshari, M Farsimadan… - Biotechnology …, 2020 - Elsevier
Tag-assisted protein purification is a method of choice for both academic researches and
large-scale industrial demands. Application of the purification tags in the protein production …
large-scale industrial demands. Application of the purification tags in the protein production …
[HTML][HTML] Targeted expression, purification, and cleavage of fusion proteins from inclusion bodies in Escherichia coli
Today, proteins are typically overexpressed using solubility-enhancing fusion tags that allow
for affinity chromatographic purification and subsequent removal by site-specific protease …
for affinity chromatographic purification and subsequent removal by site-specific protease …
Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems?
Recombinant DNA (rDNA) technologies allow the production of a wide range of peptides,
proteins and metabolites from naturally non-producing cells. Since human insulin was the …
proteins and metabolites from naturally non-producing cells. Since human insulin was the …
Perspectives of inclusion bodies for bio-based products: curse or blessing?
The bacterium Escherichia coli is a major host for recombinant protein production of non-
glycosylated products. Depending on the expression strategy, the recombinant protein can …
glycosylated products. Depending on the expression strategy, the recombinant protein can …
Self-cleaving fusion tags for recombinant protein production
Y Li - Biotechnology letters, 2011 - Springer
Fusion expression is a common practice for recombinant protein production. Some fusion
tags confer solubility on the target protein whereas others provide affinity handles that …
tags confer solubility on the target protein whereas others provide affinity handles that …
An insight into fusion technology aiding efficient recombinant protein production for functional proteomics
Advancements in peptide fusion technologies to maximize the protein production has taken
a big leap to fulfill the demands of post-genomics era targeting elucidation of …
a big leap to fulfill the demands of post-genomics era targeting elucidation of …