The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

Synthetic Biology is a rapidly growing interdisciplinary field that is primarily built upon
foundational advances in molecular biology combined with engineering design principles …

We have developed a protocol to assemble in one step and one tube at least nine separate
DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained …

2017-02-28 Assigned to Zymergen, Inc. reassignment Zymergen, Inc. ASSIGNMENT OF
ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: SHELLMAN, ERIN …

DNA assembly methods are essential tools for biological research and biotechnology.
Therefore various methods have been developed to clone DNA fragments of interest …

Methods for the cloning of large numbers of open reading frames into expression vectors are
of critical importance for challenging structural biology projects. Here we describe a system …

Fluorescent protein (FP)-based Förster resonance energy transfer (FRET) technology is
useful for development of functional indicators to visualize second messenger molecules …

Current standard cloning methods based on the use of restriction enzymes and ligase are
very versatile, but are not well suited for high-throughput cloning projects or for assembly of …

Terpene synthases catalyze the formation of a variety of terpene chemical structures.
Systematic mutagenesis studies have been effective in providing insights into the …

A basic requirement for synthetic biology is the availability of efficient DNA assembly
methods. We have previously reported the development of Golden Gate cloning, a method …