Soil protists: a fertile frontier in soil biology research
Protists include all eukaryotes except plants, fungi and animals. They are an essential, yet
often forgotten, component of the soil microbiome. Method developments have now …
often forgotten, component of the soil microbiome. Method developments have now …
Antibiotic resistance in wastewater treatment plants: Tackling the black box
Wastewater is among the most important reservoirs of antibiotic resistance in urban
environments. The abundance of carbon sources and other nutrients, a variety of possible …
environments. The abundance of carbon sources and other nutrients, a variety of possible …
[HTML][HTML] Establishing what constitutes a healthy human gut microbiome: state of the science, regulatory considerations, and future directions
MI McBurney, C Davis, CM Fraser, BO Schneeman… - The Journal of …, 2019 - Elsevier
ABSTRACT On December 17, 2018, the North American branch of the International Life
Sciences Institute (ILSI North America) convened a workshop “Can We Begin to Define a …
Sciences Institute (ILSI North America) convened a workshop “Can We Begin to Define a …
[HTML][HTML] Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling
The use of relative abundance data from next generation sequencing (NGS) can lead to
misinterpretations of microbial community structures, as the increase of one taxon leads to …
misinterpretations of microbial community structures, as the increase of one taxon leads to …
Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story?
It is well known that healthy gut microbiota is essential to promote host health and well‐
being. The intestinal microbiota of endothermic animals as well as fish are classified as …
being. The intestinal microbiota of endothermic animals as well as fish are classified as …
[HTML][HTML] Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
Background The study of microbial communities has been revolutionised in recent years by
the widespread adoption of culture independent analytical techniques such as 16S rRNA …
the widespread adoption of culture independent analytical techniques such as 16S rRNA …
Accurate estimation of fungal diversity and abundance through improved lineage-specific primers optimized for Illumina amplicon sequencing
While high-throughput sequencing methods are revolutionizing fungal ecology, recovering
accurate estimates of species richness and abundance has proven elusive. We sought to …
accurate estimates of species richness and abundance has proven elusive. We sought to …
Choice of capture and extraction methods affect detection of freshwater biodiversity from environmental DNA
Environmental DNA (eDNA) is used to detect biodiversity by the capture, extraction, and
identification of DNA shed to the environment. However, eDNA capture and extraction …
identification of DNA shed to the environment. However, eDNA capture and extraction …
Who is eating what: diet assessment using next generation sequencing
F Pompanon, BE Deagle, WOC Symondson… - Molecular …, 2012 - Wiley Online Library
The analysis of food webs and their dynamics facilitates understanding of the mechanistic
processes behind community ecology and ecosystem functions. Having accurate techniques …
processes behind community ecology and ecosystem functions. Having accurate techniques …
The choice of universal primers and the characteristics of the species mixture determine when DNA metabarcoding can be quantitative
DNA metabarcoding is a technique used to survey biodiversity in many ecological settings,
but there are doubts about whether it can provide quantitative results, that is, the proportions …
but there are doubts about whether it can provide quantitative results, that is, the proportions …